Novel peptides with HIV-1 reverse transcriptase inhibitory activity derived from the fruits ofQuercus infectoria

被引：7
作者：
Seetaha, Supaphorn [1 ]
Hannongbua, Supa [2 ]
Rattanasrisomporn, Jatuporn [1 ,3 ]
Choowongkomon, Kiattawee [4 ,5 ]
机构：
[1] Kasetsart Univ, Inst Adv Studies, Ctr Adv Studies Agr & Food, Bangkok, Thailand
[2] Kasetsart Univ, Fac Sci, Dept Chem, Bangkok, Thailand
[3] Kasetsart Univ, Fac Vet Med, Dept Compan Anim Clin Sci, Bangkok 10900, Thailand
[4] Kasetsart Univ, Fac Sci, Dept Biochem, Bangkok 10900, Thailand
[5] Kasetsart Univ, Omics Ctr Agr Bioresources Food & Hlth, Bangkok, Thailand
来源：
CHEMICAL BIOLOGY & DRUG DESIGN | 2021年 / 97卷 / 01期
关键词：
HIV-1 reverse transcriptase; mass spectrophotometer; plant peptides; Quercusinfectoria; Thai-medicinal plant; EXTRACTS; PROTEIN;
D O I：
10.1111/cbdd.13770
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The HIV-1 reverse transcriptase (HIV-1 RT), which is responsible for transcription of viral RNA genomes into DNA genomes, has become an important target for the treatment of patients with HIV infection. Hydrolyzed peptides from plants are considered a new source of potential drugs. In order to develop new effective inhibitors, peptides extracted from 111 Asian medicinal plants were screened against the HIV-1 RT. The crude hydrolyzed peptides from the fruit peel ofQuercus infectoriawere selected for purification and peptide sequence determination by HPLC and LC-MS. Two peptides of interest were synthesized, and an IC(50)test was performed to determine their ability to inhibit the HIV-1 RT. The IC(50)values of the peptides AIHIILI and LIAVSTNIIFIVV were determined to be 274 +/- 5.10 nmand 236.4 +/- 7.07 nm, respectively. This indicated that these peptides could be further developed as potential HIV-1 RT inhibitors.
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收藏
页码：157 / 166
页数：10
相关论文
共 36 条
[1]   Improving the antibacterial activity and selectivity of an ultra short peptide by hydrophobic and hydrophilic amino acid stretches [J].
Anunthawan, Thitiporn ;
Yaraksa, Nualyai ;
Phosri, Santi ;
Theansungnoen, Tinnakorn ;
Daduang, Sakda ;
Dhiravisit, Apisak ;
Thammasirirak, Sompong .
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2013, 23 (16) :4657-4662
[2]  
CHANG RS, 1991, P SOC EXP BIOL MED, V197, P59
[3]   HIV-1 integrase interacts with yeast microtubule-associated proteins [J].
de Soultrait, VR ;
Caumont, A ;
Durrens, P ;
Calmels, C ;
Parissi, V ;
Recordon, P ;
Bon, E ;
Desjobert, C ;
Tarrago-Litvak, L ;
Fournier, M .
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2002, 1575 (1-3) :40-48
[4]  
El Annaz H, 2012, AIDS RES HUM RETROV, V28, P944, DOI [10.1089/aid.2011.0278, 10.1089/AID.2011.0278]
[5]   Strategies to stabilize cell penetrating peptides for in vivo applications [J].
Fominaya, Jesus ;
Bravo, Jeronimo ;
Rebollo, Angelita .
THERAPEUTIC DELIVERY, 2015, 6 (10) :1171-1194
[6]   Peptides derived from the reverse transcriptase of human immunodeficiency virus type 1 as novel inhibitors of the viral integrase [J].
Gleenberg, IO ;
Avidan, O ;
Goldgur, Y ;
Herschhorn, A ;
Hizi, A .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (23) :21987-21996
[7]   Inhibitory effect of aqueous dandelion extract on HIV-1 replication and reverse transcriptase activity [J].
Han, Huamin ;
He, Wen ;
Wang, Wei ;
Gao, Bin .
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2011, 11
[8]   Cyclic peptide inhibitors of HIV-1 integrase derived from the LEDGF/p75 protein [J].
Hayouka, Zvi ;
Hurevich, Mattan ;
Levin, Aviad ;
Benyamini, Hadar ;
Iosub, Anat ;
Maes, Michal ;
Shalev, Deborah E. ;
Loyter, Abraham ;
Gilon, Chaim ;
Friedler, Assaf .
BIOORGANIC & MEDICINAL CHEMISTRY, 2010, 18 (23) :8388-8395
[9]   HIV-1 Reverse Transcription [J].
Hu, Wei-Shau ;
Hughes, Stephen H. .
COLD SPRING HARBOR PERSPECTIVES IN MEDICINE, 2012, 2 (10)
[10]   HIV inhibitor from Thai bitter gourd [J].
Jiratchariyakul, W ;
Wiwat, C ;
Vongsakul, M ;
Somanabandhu, A ;
Leelamanit, W ;
Fujii, I ;
Suwannaroj, N ;
Ebizuka, Y .
PLANTA MEDICA, 2001, 67 (04) :350-353
1234