Retracing the in vivo haematopoietic tree using single-cell methods

被引:12
作者
Perie, Leila [1 ,2 ]
Duffy, Ken R. [3 ]
机构
[1] PSL Res Univ, CNRS UMR168, Inst Curie, 26 rue Ulm, F-75005 Paris, France
[2] UPMC Univ Paris 06, Sorbonne Univ, Paris, France
[3] Maynooth Univ, Hamilton Inst, Maynooth, Kildare, Ireland
基金
爱尔兰科学基金会;
关键词
barcoding; haematopoietic tree; lineage tracing; mass cytometry; RNA-sequencing; single cells; GENE-EXPRESSION ANALYSIS; STEM-CELLS; CLONAL ANALYSIS; LINEAGE ANALYSIS; DIFFERENTIATION; HETEROGENEITY; DYNAMICS; TRACKING; REVEALS; RECONSTITUTION;
D O I
10.1002/1873-3468.12299
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The dynamic process by which self-renewing stem cells and their offspring proliferate and differentiate to create the erythroid, myeloid and lymphoid lineages of the blood system has long since been an important topic of study. A range of recent single cell and family tracing methodologies such as massively parallel single-cell RNA-sequencing, mass cytometry, integration site barcoding, cellular barcoding and transposon barcoding are enabling unprecedented analysis, dissection and re-evaluation of the haematopoietic tree. In addition to the substantial experimental advances, these new techniques have required significant theoretical development in order to make biological deductions from their data. Here, we review these approaches from both an experimental and inferential point of view, considering their discoveries to date, their capabilities, limitations and opportunities for further development.
引用
收藏
页码:4068 / 4083
页数:16
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