Two-photon excited fluorescence of silica nanoparticles loaded with a fluorene-based monomer and its cross-conjugated polymer: their application to cell imaging

被引：34

作者：

Aparicio-Ixta, Laura ^{[1
]}

Ramos-Ortiz, Gabriel ^{[1
]}

Pichardo-Molina, Juan L. ^{[1
]}

Luis Maldonado, Jose ^{[1
]}

Rodriguez, Mario ^{[1
]}

Tellez-Lopez, Victor M. ^{[2
]}

Martinez-Fong, Daniel ^{[2
,3
]}

Zolotukhin, Mikhail G. ^{[4
]}

Fomine, Serguei ^{[4
]}

Meneses-Nava, Marco. A. ^{[1
]}

Barbosa-Garcia, Oracio ^{[1
]}

机构：

[1] Ctr Invest Opt AC, Leon 37000, Gto, Mexico

[2] CINVESTAV, Dept Fisiol Biofis & Neurociencias, Mexico City 07000, DF, Mexico

[3] CINVESTAV, Programa Doctorado Nanociencias & Nanotecnol, Mexico City 07000, DF, Mexico

[4] Univ Nacl Autonoma Mexico, Inst Invest Mat, Mexico City 04510, DF, Mexico

来源：

NANOSCALE | 2012年 / 4卷 / 24期

关键词：

LIGHT-EMITTING-DIODES; ABSORPTION; EXCITATION; MOLECULES; SECTIONS;

D O I：

10.1039/c2nr31925j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this work the two-photon activity of nanoparticles obtained from a fluorene monomer (M1) and its cross-conjugated polymer (P1) is reported. Aqueous suspensions of M1 and P1 nanoparticles prepared through the reprecipitation method exhibited maximum two-photon absorption (TPA) cross-sections of 84 and 9860 GM (1 GM = 10(-50) cm(4) s) at 740 nm, respectively, and a fluorescence quantum yield of similar to 1. Such a two-photon activity was practically equal with respect to that for molecular solutions of M1 and P1. These materials were then successfully encapsulated into silica nanoparticles to provide biocompatibly. A lung cancer cell line (A549) and a human cervical cancer cell line (HeLa cells) were incubated with our fluorescent silica nanoparticles to carry out two-photon imaging. By means of these studies we demonstrate that optimized nonlinear optical polymers loaded in silica nanoparticles can be used as efficient probes with low cytotoxicity and good photostability for two-photon fluorescence microscopy. To the best of our knowledge, studies concerning polymer-doped silica nanoparticles exhibiting large two-photon activity have not been reported in the literature.

引用

页码：7751 / 7759

页数：9

共 47 条

[1] Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm [J].