Recruitment of the SWI/SNF chromatin remodeling complex by transcriptional activators

被引：200
作者：
Yudkovsky, N
Logie, C
Hahn, S
Peterson, CL [1 ]
机构：
[1] Univ Massachusetts, Med Ctr, Program Mol Med, Worcester, MA 01605 USA
[2] Univ Massachusetts, Med Ctr, Dept Biochem & Mol Biol, Worcester, MA 01605 USA
[3] Fred Hutchinson Canc Res Ctr, Seattle, WA 98109 USA
[4] Howard Hughes Med Inst, Seattle, WA 98109 USA
[5] Univ Washington, Mol & Cellular Biol Program, Fred Hutchinson Canc Res Ctr, Seattle, WA 98195 USA
来源：
GENES & DEVELOPMENT | 1999年 / 13卷 / 18期
关键词：
SWI-SNF; chromatin; transcription gene regulation; holoenzyme;
D O I：
10.1101/gad.13.18.2369
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
SWI/SNF is a chromatin remodeling complex that facilitates expression of a number of yeast genes. Here we demonstrate that SWI/SNF can be recruited from yeast nuclear extracts by a transcriptional activator. Recruitment is dependent on an activation domain but not on promoter sequences, TBP, or RNA polymerase II holoenzyme. We also show that acidic activation domains can target SWI/SNF remodeling activity. These results demonstrate that SWI/SNF activity can be targeted by gene-specific activators and that this recruitment can occur independently of Pol II holoenzyme.
引用
收藏
页码：2369 / 2374
页数：6
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