Use of fluorescence to monitor the incorporation of horseradish peroxidase into a sol-gel derived medium

被引：22

作者：

Hungerford, G ^{[1
]}

Rei, A ^{[1
]}

Ferreira, MIC ^{[1
]}

机构：

[1] Univ Minho, Dept Fis, P-4710057 Braga, Portugal

来源：

BIOPHYSICAL CHEMISTRY | 2006年 / 120卷 / 02期

关键词：

nile red; energy transfer; molecular dynamics; catalytic activity;

D O I：

10.1016/j.bpc.2005.10.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The solvatochromic dye nile red has been employed to monitor the incorporation of an enzyme (horseradish peroxidase) into a sol-gel derived medium. The fluorescence spectrum of the dye, when incorporated into the enzyme, was analysed as the sum of Gaussian component spectra and relative changes between these component spectra were monitored upon encapsulation of the dye-enzyme system within the host matrix. Activity of the confined enzyme was verified and the effect of temperature was also investigated, through the examination of nile red fluorescence in the sol-gel derived matrix, where a stabilising effect was noted. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：81 / 86

页数：6

共 24 条

[1] Effects of encapsulation in sol-gel silica glass on esterase activity, conformational stability, and unfolding of bovine carbonic anhydrase II [J].