Uptake of oleoyl-chitosan nanoparticles by A549 cells

被引：59

作者：

Zhang, Jing ^{[1
]}

Chen, Xi Guang ^{[1
]}

Peng, Wei Bing ^{[2
]}

Liu, Cheng Sheng ^{[1
]}

机构：

[1] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

[2] Ocean Univ China, Coll Med & Pharm, Qingdao 266003, Peoples R China

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2008年 / 4卷 / 03期

基金：

中国国家自然科学基金;

关键词：

A549; cells; Cellular uptake; Fluorescein isothiocyanate (FITC); Nanoparticles;

D O I：

10.1016/j.nano.2008.03.006

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The aim of the present study was to evaluate cellular uptake of oleoyl-chitosan (OCH) nanoparticles by using A549 cells, a human lung carcinoma cell line, for drug and gene delivery applications. In this study, self-assembled OCH nanoparticles encapsulating a fluorescent marker molecule, fluorescein isothiocyanate (FITC), were prepared and characterized. The effects of particle size, concentration, and incubation time on the cellular uptake of the nanoparticles (FITC-OCH nanoparticles) were quantified by spectrofluorometric measurement and confirmed using fluorescence microscopy studies. The nanoparticles were taken up by the cells, and levels of binding and uptake increased with the decrease of particle size and the increase of particle concentration and incubation time. These results implied that the OCH nanoparticles have great potential to be applied as a drug carrier system to deliver drugs into the cells. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：208 / 214

页数：7

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