Effect of Y-27632 on the cultured retinal neurocytes of rats

被引：2

作者：

Feng, Pei-Li ^{[1
]}

Wang, Jing ^{[1
]}

Yang, Zi-Jian ^{[1
]}

Liu, Xiao-Hong ^{[1
]}

Zhong, Yi-Sheng ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Dept Ophthalmol, Ruijin Hosp, Sch Med, Shanghai 200025, Peoples R China

来源：

INTERNATIONAL JOURNAL OF OPHTHALMOLOGY | 2013年 / 6卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Y-27632; retinal neurocytes; cell culture; neurites; NEURITE OUTGROWTH; AXONAL REGENERATION; OPTIC-NERVE; RHO; INHIBITION; KINASE; PATHWAYS; SURVIVAL; NEURONS; GROWTH;

D O I：

10.3980/j.issn.2222-3959.2013.01.03

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

AIM: To investigate the effect of Y -27632 on the survival and neurite outgrowth of the cultured retinal neurocytes. METHODS: After the postnatal day 2 -3, Sprague - Dawley retinal neurocytes were cultured for 48 hours, the culture media was replaced with serum -free media (control group) and serum-free media contained 30 mu mol/L Y-27632 (Y-27632 group), and the cells were continually cultured another 48 hours. The cultured retinal neurocytes were identified with anti -neuron specific enolase (NSE) immunocytochemistry. The survival state of those cells was estimated by MTT assay, and the neurite outgrowth of those cells was evaluated by the computerized image-analysis system. RESULTS: Compared with the control group, the absorbance values of cells survival in Y -27632 group increased 12.90% and 33.33% respectively after 72 and 96 hours culture. Y -27632 had no significant effect on the diameter of cultured retinal neurocytes. Compared with the control group, Y -27632 induced a stable improvement of neurite outgrowth of retinal neurocytes after 72 and 96 hours culture (P=0.001). CONCLUSION: Y -27632 could promote the survival and neurite outgrowth of the early postnatal cultured retinal neurocytes.

引用

页码：15 / 18

页数：4

共 15 条

[1] ROCK inhibition promotes adult retinal ganglion cell neurite outgrowth only in the presence of growth promoting factors [J].