In vitro antiproliferative effect of a water-soluble Laminaria japonica polysaccharide on human melanoma cell line A375

A water-soluble polysaccharide WPS-2-1, purified from Laminaria japonica, has been found to have antitumor activity. In this study, WPS-2-1 exhibited high anti-proliferative activity on A375 cells in a dose-dependent manner. Further investigation indicated that WPS-2-1 induced A375 cells apoptosis. Moreover, WPS-2-1-induced apoptosis was associated with the alteration in expressions of Bcl-2 family proteins. Mitochonadrial apoptotic pathway was involved in WPS-2-1-induced apoptosis, which included the loss of mitochondrial membrane and activation of caspase-3/9. The results in this study suggested that WPS-2-1 could effectively inhibit proliferation of A375 cells in vitro and induce apoptosis via mitochondrial apoptotic pathway. It might serve as a potential antitumor agent. (C) 2013 Elsevier Ltd. All rights reserved.