Graphene and CdS nanocomposite: A facile interface for construction of DNA-based electrochemical biosensor and its application to the determination of phenformin

被引：25
作者：
Zeng, Lijiao [1 ]
Wang, Rui [1 ]
Zhu, Lihua [1 ]
Zhang, Jingdong [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Wuhan 430074, Peoples R China
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2013年 / 110卷
基金：
国家高技术研究发展计划(863计划); 中国国家自然科学基金;
关键词：
Graphene/CdS; Nanocomposite; DNA; Electrochemical biosensor; Phenformin; MODIFIED ELECTRODE; METHYLENE-BLUE; DAMAGE; ADSORPTION; SURFACES; OLIGONUCLEOTIDES; IMMOBILIZATION; HYDROCHLORIDE; NANOCRYSTAL; GENOSENSOR;
D O I：
10.1016/j.colsurfb.2013.04.025
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Graphene/cadmium sulphide (GR-CdS) nanocomposite was synthesized via a low temperature process in aqueous solution. The as-prepared nanocomposite was characterized by scanning electron microscopy, UV-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. The impedance analysis indicated that GR-CdS nanocomposite possessed outstanding electrochemical performance for facile electron transfer. When DNA was immobilized on GR-CdS (DNA/GR-CdS) modified electrode, the electrochemical oxidation of guanine and adenine in DNA residue bases was significantly promoted. Due to the interaction of DNA with phenformin, the voltammetric current of guanine or adenine on the DNA/GR-CdS electrode was decreased when phenformin was present in the electrolytic solution. Under optimized conditions, the signal of guanine on DNA/GR-CdS electrode decreased linearly with increasing the concentration of phenformin in the range of 1.0 x 10(-6) mol L-1 to 1.0 x 10(-3) mol L-1. The proposed DNA-based electrochemical biosensor was successfully applied to the determination of phenformin in real samples. (c) 2013 B.V. All rights reserved.
引用
收藏
页码：8 / 14
页数：7
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