Graphene and CdS nanocomposite: A facile interface for construction of DNA-based electrochemical biosensor and its application to the determination of phenformin

被引：25
作者：
Zeng, Lijiao [1 ]
Wang, Rui [1 ]
Zhu, Lihua [1 ]
Zhang, Jingdong [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Sch Chem & Chem Engn, Wuhan 430074, Peoples R China
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2013年 / 110卷
基金：
国家高技术研究发展计划(863计划); 中国国家自然科学基金;
关键词：
Graphene/CdS; Nanocomposite; DNA; Electrochemical biosensor; Phenformin; MODIFIED ELECTRODE; METHYLENE-BLUE; DAMAGE; ADSORPTION; SURFACES; OLIGONUCLEOTIDES; IMMOBILIZATION; HYDROCHLORIDE; NANOCRYSTAL; GENOSENSOR;
D O I：
10.1016/j.colsurfb.2013.04.025
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Graphene/cadmium sulphide (GR-CdS) nanocomposite was synthesized via a low temperature process in aqueous solution. The as-prepared nanocomposite was characterized by scanning electron microscopy, UV-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. The impedance analysis indicated that GR-CdS nanocomposite possessed outstanding electrochemical performance for facile electron transfer. When DNA was immobilized on GR-CdS (DNA/GR-CdS) modified electrode, the electrochemical oxidation of guanine and adenine in DNA residue bases was significantly promoted. Due to the interaction of DNA with phenformin, the voltammetric current of guanine or adenine on the DNA/GR-CdS electrode was decreased when phenformin was present in the electrolytic solution. Under optimized conditions, the signal of guanine on DNA/GR-CdS electrode decreased linearly with increasing the concentration of phenformin in the range of 1.0 x 10(-6) mol L-1 to 1.0 x 10(-3) mol L-1. The proposed DNA-based electrochemical biosensor was successfully applied to the determination of phenformin in real samples. (c) 2013 B.V. All rights reserved.
引用
收藏
页码：8 / 14
页数：7
相关论文
共 47 条
  • [1] Detection of the damage caused to DNA by niclosamide using an electrochemical DNA-biosensor
    Abreu, FC
    Goulart, MOF
    Brett, AMO
    [J]. BIOSENSORS & BIOELECTRONICS, 2002, 17 (11-12) : 913 - 919
  • [2] Glassy carbon electrodes modified with CNT dispersed in chitosan: Analytical applications for sensing DNA-methylene blue interaction
    Arias, Paulina
    Ferreyra, Nancy F.
    Rivas, Gustavo A.
    Bollo, Soledad
    [J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2009, 634 (02) : 123 - 126
  • [3] Phenformin has a direct inhibitory effect on the ATP-sensitive potassium channel
    Aziz, Qadeer
    Thomas, Alison
    Khambra, Tapsi
    Tinker, Andrew
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 2010, 634 (1-3) : 26 - 32
  • [4] Electrocatalytic voltammetric determination of guanine at a cobalt phthalocyanine modified carbon nanotubes paste electrode
    Balan, Ionela
    David, Iulia Gabriela
    David, Vasile
    Stoica, Anca-Iulia
    Mihailciuc, Constantin
    Stamatin, Ioan
    Ciucu, Anton Alexandru
    [J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2011, 654 (1-2) : 8 - 12
  • [5] A novel electrochemical DNA biosensor based on graphene and polyaniline nanowires
    Bo, Yang
    Yang, Huiyan
    Hu, Ying
    Yao, Tianming
    Huang, Shasheng
    [J]. ELECTROCHIMICA ACTA, 2011, 56 (06) : 2676 - 2681
  • [6] Effect of pH and applied potential on the adsorption of DNA on highly oriented pyrolytic graphite electrodes. Atomic force microscopy surface characterisation
    Brett, AMO
    Chiorcea, AM
    [J]. ELECTROCHEMISTRY COMMUNICATIONS, 2003, 5 (02) : 178 - 183
  • [7] Cu@Au alloy nanoparticle as oligonucleotides labels for electrochemical stripping detection of DNA hybridization
    Cai, H
    Zhu, NN
    Jiang, Y
    He, PG
    Fang, YZ
    [J]. BIOSENSORS & BIOELECTRONICS, 2003, 18 (11) : 1311 - 1319
  • [8] A Facile One-step Method to Produce Graphene-CdS Quantum Dot Nanocomposites as Promising Optoelectronic Materials
    Cao, Aoneng
    Liu, Zhen
    Chu, Saisai
    Wu, Minghong
    Ye, Zhangmei
    Cai, Zhengwei
    Chang, Yanli
    Wang, Shufeng
    Gong, Qihuang
    Liu, Yuanfang
    [J]. ADVANCED MATERIALS, 2010, 22 (01) : 103 - +
  • [9] Effects of phenformin on the proliferation of human tumor cell lines
    Caraci, F
    Chisari, M
    Frasca, G
    Chiechio, S
    Salomone, S
    Pinto, A
    Sortino, MA
    Bianchi, A
    [J]. LIFE SCIENCES, 2003, 74 (05) : 643 - 650
  • [10] The interaction of water-soluble iron porphyrins with DNA films and the electrocatalytic properties for inorganic and organic nitro compounds
    Chen, SM
    Chen, SV
    [J]. ELECTROCHIMICA ACTA, 2003, 48 (27) : 4049 - 4060
12345