A Phenylbutenoid Dimer, cis-3-(3′,4′-Dimethoxyphenyl)-4-[(E)-3′′′,4′′′-Dimethoxystyryl]Cyclohex-1-ene, Exhibits Apoptogenic Properties in T-Acute Lymphoblastic Leukemia Cells via Induction of p53-Independent Mitochondrial Signalling Pathway

被引：14

作者：

Anasamy, Theebaa ^{[1
]}

Abdul, Ahmad Bustamam ^{[1
]}

Sukari, Mohd Aspollah ^{[2
]}

Abdelwahab, Siddig Ibrahim ^{[3
,4
]}

Mohan, Syam ^{[3
]}

Kamalidehghan, Behnam ^{[3
]}

Azid, Mohd Zulkhairi ^{[2
]}

Nadzri, Nabilah Muhammad ^{[1
]}

Andas, A. Reenaa Joys ^{[1
]}

Beng, Ng Kuan ^{[1
]}

Hadi, A. Hamid A. ^{[5
]}

Rahman, Heshu Sulaiman ^{[1
,6
]}

机构：

[1] Univ Putra Malaysia, UPM MAKNA Canc Res Lab, Inst Biosci, Serdang 43400, Selangor, Malaysia

[2] Univ Putra Malaysia, Dept Chem, Fac Sci, Serdang 43400, Selangor, Malaysia

[3] Univ Malaya, Fac Med, Dept Pharm, Kuala Lumpur 50603, Malaysia

[4] Jazan Univ, Med Res Ctr, Fac Med, Jazan, Saudi Arabia

[5] Univ Malaya, Fac Sci, Kuala Lumpur 50603, Malaysia

[6] Univ Putra Malaysia, Dept Pathol & Microbiol, Fac Vet Med, Upm Serdang 43400, Selangor, Malaysia

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2013年 / 2013卷

关键词：

HEAT-SHOCK-PROTEIN-70 INHIBITS APOPTOSIS; S-PHASE ARREST; DNA FRAGMENTATION; MEDICINAL-PLANTS; DRUG DISCOVERY; PROLIFERATION; CYTOTOXICITY; EXPRESSION; GROWTH; RHODAMINE-123;

D O I：

10.1155/2013/939810

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

The current study was designed to evaluate the in vitro cytotoxicity effect of a phenylbutenoid dimer, cis-3-(3',4'-dimethoxyphenyl)4-[(E)-3"',4"'-dimethoxystyryl]cyclohex-1-ene (ZC-B11) isolated from the rhizome of Zingiber cassumunar on various cancer cell line, and normal human blood mononuclear cells, and to further investigate the involvement of apoptosis-related proteins that leads, to the probable pathway in which apoptosis is triggered. Cytotoxicity test using MTT assay showed selective inhibition of ZC-B11 towards T-acute lymphoblastic leukemia cells, CEMss, with an IC50 value of 7.11 +/- 0.240 mu g/mL, which did not reveal cytotoxic effects towards normal human blood mononuclear cells (IC50 > 50 mu g/mL). Morphology assessments demonstrated distinctive morphological changes corresponding to a typical apoptosis. ZC-B11 also arrested cell cycle progression at S phase and causes DNA fragmentation in CEMss cells. Decline of mitochondrial membrane potential was also determined qualitatively. In the apoptosis-related protein determination, ZC-B11 was found to significantly upregulate Bax, caspase 3/7, caspase 9, cytochrome c, and SMAC and downregulate Bcl-2, HSP70, and XIAP, but did not affect caspase 8, p53, and BID. These results demonstrated for the first time the apoptogenic property of ZC-B11 on CEMss cell line, leading to the programmed cell death via intrinsic mitochondrial pathway of apoptosis induction.

引用

页数：14

共 37 条

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