Structural studies of the O-antigenic polysaccharide of the bovine mastitis isolate Escherichia coli serotype O174

被引：5
作者：
Duda, Katarzyna A. [1 ]
Fruth, Angelika [2 ]
Holst, Otto [1 ]
机构：
[1] Airway Res Ctr North, Res Ctr Borstel, Leibniz Ctr Med & Biosci, Div Struct Biochem, D-23845 Borstel, Germany
[2] NRZ Salmonellen & Andere Bakterielle Enteritiserr, D-38855 Wernigerode, Germany
来源：
CARBOHYDRATE RESEARCH | 2013年 / 373卷
关键词：
Escherichia coli; Mastitis; O-Antigen; NMR spectroscopy; Serotype O174;
D O I：
10.1016/j.carres.2013.03.004
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Escherichia coli is one of the major causative agents of bovine mastitis, a disease affecting dairy herds. The lipopolysaccharide (LPS) of E. coli was plays a prominent role during infection. Here, we report on the O-antigen chemical structure of the LPS from Escherichia coli strain 2188 (serotype O174:H28) isolated from a mastitis-diseased cow. The structure of the OPS was analyzed by 1 and 2D NMR spectroscopy, and methylation analysis, which identified the branched repeating tetrasaccharide biological unit. beta-D-GlcpNAc 1 down arrow 2 -> 4)-beta-D-GlcpA-(1 -> 3)-beta-D-Galp-(1 -> 3)-beta-D-GlcpNAc-(1 -> (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：18 / 21
页数：4
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