Molecularly Engineered "Janus GroEL": Application to Supramolecular Copolymerization with a Higher Level of Sequence Control

被引:14
作者
Kashiwagi, Daiki [1 ]
Shen, Hao K. [1 ]
Sim, Seunghyun [3 ]
Sano, Koki [2 ]
Ishida, Yasuhiro [2 ]
Kimura, Ayumi [4 ]
Niwa, Tatsuya [5 ]
Taguchi, Hideki [5 ]
Aida, Takuzo [1 ,2 ]
机构
[1] Univ Tokyo, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan
[2] RIKEN, Ctr Emergent Matter Sci, Wako, Saitama 3510198, Japan
[3] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA
[4] Univ Tokyo, Inst Engn Innovat, Bunkyo Ku, Tokyo 1138656, Japan
[5] Tokyo Inst Technol, Inst Innovat Res, Cell Biol Ctr, Yokohama, Kanagawa 2268503, Japan
关键词
POLYMERIC MATERIALS; CRYSTAL-STRUCTURE; CHAPERONIN GROEL; PROTEIN; DNA; EXCHANGE; NANOPARTICLES; RELEASE; NANOSTRUCTURES; STABILIZATION;
D O I
10.1021/jacs.0c05937
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Herein we report the synthesis and isolation of a shape-persistent Janus protein nanoparticle derived from the biomolecular machine chaperonin GroEL ((A)GroEL(B)) and its application to DNA-mediated ternary supramolecular copolymerization. To synthesize (A)GroEL(B) with two different DNA strands A and B at its opposite apical domains, we utilized the unique biological property of GroEL, i.e., Mg2+/ATP-mediated ring exchange between (A)GroEL(A) and (B)GroEL(B) with their hollow cylindrical double-decker architectures. This exchange event was reported more than 24 years ago but has never been utilized for molecular engineering of GroEL. We leveraged DNA nanotechnology to purely isolate Janus A GroEL B and succeeded in its precision ternary supramolecular copolymerization with two DNA comonomers, A** and B*, that are partially complementary to A and B in (A)GroEL(B), respectively, and programmed to self-dimerize on the other side. Transmission electron microscopy allowed us to confirm the formation of the expected dual-periodic copolymer sequence -((B)*(/B)GroEL(A/A)**(/A)**(/A)GroEL(B/B)*)- in the form of a laterally connected lamellar assembly rather than a single-chain copolymer.
引用
收藏
页码:13310 / 13315
页数:6
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