Identification of novel genes with prognostic value in childhood leukemia using cDNA microarray and quantitative RT-PCR

被引：9
作者：
Hattori, Hiroyoshi
Matsuzaki, Akinobu
Suminoe, Aiko
Koga, Yuhki
Tahiro, Kosuke
Hara, Toshiro
机构：
[1] Kyushu Univ, Grad Sch Med Sci, Dept Pediat, Higashi Ku, Fukuoka 8128582, Japan
[2] Kyushu Univ, Sch Hlth Sci, Div Child Hlth, Fukuoka 8128582, Japan
[3] Kyushu Univ, Sch Med Sci, Dept Pediat, Fukuoka 8128582, Japan
[4] Kyushu Univ, Grad Sch Genet Resources Technol, Fukuoka 8128582, Japan
来源：
PEDIATRIC HEMATOLOGY AND ONCOLOGY | 2006年 / 23卷 / 02期
关键词：
CASP2 and RIPK1 domain containing adaptor with death domain (CRADD); cDNA microarray; cyclin-dependent kinase inhibitor (CDKN2C); insulin-like growth factor binding protein 2 (IGFBP-2); prognostic marker; quantitative RT-PCR;
D O I：
10.1080/08880010500457780
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The aim, of this study was to identify genes distinctively expressed or suppressed in. childhood leukemia with different prognoses using c-DNA microarray and quantitative reverse transcription-polymerase chain reaction, (RT-PCR). The expression levels of the selected genes by cDNA microarray were quantified in primary leukemic blasts from 44 patients (acute lymphoblastic leukemia, 28; acute myelogenous leukemia (AML), 13; transient myeloproliferative disorder, 3). The expression levels of CDKN2C, CRADD, and IGFBP-2 genes were significantly associated with the event-free survival of the patients in AML. The present results suggest that a combination of cDNA microarroy and quantitative RT-PCR may be useful to identify novel genes with prognostic value in childhood AML.
引用
收藏
页码：115 / 127
页数：13
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