Cryopreservation of boar semen and its future importance to the industry

被引:124
作者
Bailey, Janice L. [1 ]
Lessard, Christian [1 ]
Jacques, Joannie [1 ]
Breque, Christelle [1 ]
Dobrinski, Ina [2 ]
Zeng, Wenxian [2 ]
Galantino-Homer, Hannah L. [2 ]
机构
[1] Univ Laval, Ctr Rech Biol Reprod, Dept Anim Sci, Quebec City, PQ G1V 0A6, Canada
[2] Univ Penn, Sch Vet Med, Dept Clin Studies,New Bolton Ctr, Ctr Anim transgenesis & Germ Cell Res, Kennett Sq, PA 19348 USA
关键词
Sperm; Cold shock; Cyclodextrin; Cholesterol; Capacitation;
D O I
10.1016/j.theriogenology.2008.06.014
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Whereas AI has arguably been the most important management tool leading to improved herd productivity, long-term storage of semen brings forth additional advantages to producers of agriculturally important animals and at the AI industry. Semen cryopreservation greatly facilitates the distribution of agriculturally important animals and the AI industry. Semen cryopreservation greatly facilitates the distribution of agriculturally desirable genes, rapidly increasing herd productivity. Of particular importance to the pig industry, the use of frozen semen would help to control transmission of certain pathogens, thereby protecting the health status of the herd. Moreover, a reserve of cryopreserved semen would minimize the effects of a sudden outbreak of a contagious illness or a natural disaster. Successful cryopreservation of boar semen is necessary for international sales. Finally, effective gene banking depends on the availability of functional, cryopreserved germplasm. Despite these potential advantages of long-term semen storage, poreine sperm are notoriously sensitive to cold temperatures, and frozen-thawed semen is not routinely used by the industry. The objective of our laboratories is to develop protocols for efficienty long-term storage of porcine semen using cryopreservation. We hypothesize that since the sperm plasma membrane is the primary site of cold-induced damageg, reinforcing the membranes with molecules having particular properties, such as cholesterol, will improve the ability of boar sperm to withstand cold temperatures and cryopreservation protocols. Based on our data, such approaches should help alleviate the problems with sperm function after cooling, thereby resulting in better survival and motility characteristics, and reduced non-regulated capacitation and spontaneous acrosome reactions. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:1251 / 1259
页数:9
相关论文
共 75 条
[1]   Birth of piglets preselected for gender following in vitro fertilization of in vitro matured pig oocytes by X and Y chromosome bearing spermatozoa sorted by high speed flow cytometry [J].
Abeydeera, LR ;
Johnson, LA ;
Welch, GR ;
Wang, WH ;
Boquest, AC ;
Cantley, TC ;
Rieke, A ;
Day, BN .
THERIOGENOLOGY, 1998, 50 (07) :981-988
[2]  
ALMLID T, 1988, J ANIM SCI, V66, P2899
[3]  
BABA T, 1994, J BIOL CHEM, V269, P10133
[4]   Semen cryopreservation: Successes and persistent problems in farm species [J].
Bailey, J ;
Morrier, A ;
Cormier, N .
CANADIAN JOURNAL OF ANIMAL SCIENCE, 2003, 83 (03) :393-401
[5]  
Bailey JL, 2000, J ANDROL, V21, P1
[6]   Use of phosphoproteomics to study tyrosine kinase activity in capacitating boar sperm Kinase activity and capacitation [J].
Bailey, JL ;
Tardif, S ;
Dubé, C ;
Beaulieu, M ;
Reyes-Moreno, C ;
Lefièvre, L ;
Leclerc, P .
THERIOGENOLOGY, 2005, 63 (02) :599-614
[7]   REGULATION OF INTERNAL CA2+ BY CHILLED BULL AND BOAR SPERMATOZOA [J].
BAILEY, JL ;
BUHR, MM .
CRYOBIOLOGY, 1995, 32 (03) :259-269
[8]   MEMBRANE VESICULATION AS A FEATURE OF MAMMALIAN REACTION [J].
BARROS, C ;
BEDFORD, JM ;
FRANKLIN, LE ;
AUSTIN, CR .
JOURNAL OF CELL BIOLOGY, 1967, 34 (03) :C1-&
[9]   Changes in tyrosine phosphorylation associated with true capacitation and capacitation-like state in boar spermatozoa [J].
Bravo, MM ;
Aparicio, IM ;
Garcia-Herreros, M ;
Gil, MC ;
Peña, FJ ;
Garcia-Marin, LJ .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2005, 71 (01) :88-96
[10]   EFFECTS OF SEMEN PRESERVATION ON BOAR SPERMATOZOA HEAD MEMBRANES [J].
BUHR, MM ;
CANVIN, AT ;
BAILEY, JL .
GAMETE RESEARCH, 1989, 23 (04) :441-449