Verification of enzymes deterioration due to Cu(II) presence in an enhanced biological phosphorus removal system

被引：24

作者：

Tsai, Yung-Pin ^{[1
,2
]}

Tzeng, Huey-Fen ^{[3
]}

Lin, Jan-Wei ^{[1
]}

Lu, Meng-Shan ^{[1
]}

Lin, Jyun-Yen ^{[1
]}

机构：

[1] Natl Chi Nan Univ, Dept Civil Engn, Puli 545, Nantou, Taiwan

[2] CALTECH, Linde Robinson Labs, Pasadena, CA 91125 USA

[3] Natl Chi Nan Univ, Dept Appl Chem, Puli 545, Nantou, Taiwan

来源：

CHEMOSPHERE | 2013年 / 91卷 / 05期

关键词：

Polyhydroxyalkanoate (PHA); Glycogen; Cu(II); Phosphorus accumulating organism (PAO); Acetyl-CoA synthase (ACS); Polyphosphate kinase (PPK); ACTIVATED-SLUDGE; ACCUMULATING ORGANISMS; POLYPHOSPHATE KINASE; PHOSPHATE REMOVAL; ESCHERICHIA-COLI; CARBON SOURCE; HEAVY-METALS; WASTE-WATER; METABOLISM; ACETATE;

D O I：

10.1016/j.chemosphere.2012.11.080

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study experimentally demonstrated that polyphosphate accumulating organisms (PAOs) losing the abilities of anaerobically synthesizing polyhydroxyalkanoates and aerobically taking up phosphate under Cu(II) presence was due to the inhibition of enzyme activities of acetyl-CoA synthases (ACS) and polyphosphate kinase (PPK), respectively. ACS activity tests showed the apparent maximum specific activity (V-max) of ACS decreased with increasing Cu(II) concentration, revealing Cu(II) is a mixed inhibitor for ACS. Inhibition coefficients showed Cu(II) has a higher affinity for free ACS than for ACS-coenzyme A complex. PPK activity tests showed the Vmax substantially decreased with increasing Cu(II) concentration, revealing Cu(II) is also a mixed inhibitor for PPK. Inhibition coefficients showed Cu(II) more easily bound to free PPK than to PPK-Adenosine triphosphate complex. Experimental data also showed the aerobic mechanism of PAOs taking up phosphate was completely interrupted when 3 mg L-1 of Cu(II) was added. (c) 2012 Elsevier Ltd. All rights reserved.

引用

页码：602 / 607

页数：6

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