Formation of Substrate-Supported Membranes from Mixtures of Long- and Short-Chain Phospholipids

被引:26
|
作者
Morigaki, Kenichi [1 ,2 ,3 ]
Kimura, Shigeki [3 ]
Okada, Keisuke [2 ,3 ]
Kawasaki, Takashi [3 ]
Kawasaki, Kazunori [3 ]
机构
[1] Kobe Univ, Res Ctr Environm Genom, Nada Ku, Kobe, Hyogo 6578501, Japan
[2] Kobe Univ, Grad Sch Agr Sci, Nada Ku, Kobe, Hyogo 6578501, Japan
[3] Natl Inst Adv Ind Sci & Technol, Ikeda, Osaka 5638577, Japan
基金
日本学术振兴会;
关键词
LIPID VESICLE ADSORPTION; BILAYER-MEMBRANES; SOLID SUBSTRATE; PROTEINS; SURFACE; NMR; POLYMERIZATION; RECONSTITUTION; DETERGENTS; DEPOSITION;
D O I
10.1021/la300696z
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We studied the formation of substrate-supported planar phospholipid bilayers (SPBs) on glass and silica from mixtures of long- and short-chain phospholipids to assess the effects of detergent additives on SPB formation. 1,2-Hexyanoyl-sn-glycero-3-phosphocholine (DHPC-C6) and 1,2-heptanoyl-sn-glycero-3-phosphocholine (DHPC-C7) were chosen as short-chain phospholipids. 1-Palmitoyl-2-oleol-sn-glycero-3-phosphocholine (POPC) was used as a model long-chain phospholipid. Kinetic studies by quartz crystal microbalance with dissipation monitoring (QCM-D) showed that the presence of short-chain phospholipids significantly accelerated the formation of SPBs. Rapid rinsing with a buffer solution did not change the adsorbed mass on the surface if POPC/DHPC-C6 mixtures were used below the critical micelle concentration (cmc) of DHPC-C6, indicating that an SPB composed of POPC molecules remained on the surface. Fluorescence microscopy observation showed homogeneous SPBs, and the fluorescence recovery after photobleaching (FRAP) measurements gave a diffusion coefficient comparable to that for SPBs formed from POPC vesicles. However, mixtures of POPC/DHPC-C7 resulted in a smaller mass of lipid adsorption on the substrate. FRAP measurements also yielded significantly smaller diffusion coefficients, suggesting the presence of defects. The different behaviors for DHPC-C6 and DHPC-C7 point to the dual roles of detergents to enhance the formation of SPBs and to destabilize them, depending on their structures and aggregation properties.
引用
收藏
页码:9649 / 9655
页数:7
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