Short report:: Molecular cloning and characterization of a putative binding protein of Babesia caballi

被引:0
|
作者
Ikadai, H [1 ]
Takamatsu, Y
Takashiro, R
Segawa, A
Kudo, N
Igarashi, I
Oyamada, T
机构
[1] Kitasato Univ, Sch Vet Med & Anim Sci, Dept Vet Parasitol, Towada, Aomori 0348628, Japan
[2] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 080, Japan
来源
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2005年 / 73卷 / 06期
关键词
D O I
10.4269/ajtmh.2005.73.1135
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
A composite 2,206 nucleotide DNA sequence encoding a putative immunoglobulin-binding protein (BiP) was constructed from a sequence obtained from Babesia caballi cDNA library clones. The 1,962 nucleotide open reading frame predicts a 72 kD protein with extensive homology with BiPs from Apicomplexa parasites. The BiP gene had a predicted N-terminal signal sequence of IS amino acids and a C-terminal tetrapeptide sequence (Ser-Asp-Glu-Leu) for signaling in the endoplasmic reticulum lumen. The recombinant protein expressed in baculovirus showed an apparent mass of 72 kD, which is identical to that of the native B. caballi protein. Monoclonal antibodies (MAbs) against B. caballi BiP reacted strongly with extracellular merozoites, but not in early intraerythrocytic stage. Detailed observation showed that the reaction of MAbs against pear-shaped forms was markedly irregular, with either no reaction, or reaction with one or two brightly fluorescent pear-shaped forms (two parasites) of B. caballi.
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页码:1135 / 1138
页数:4
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