Exploring microbial community structure and biological function in manured soil during ten repeated treatments with chlortetracycline and ciprofloxacin

被引：15

作者：

Han, Lingxi ^{[1
]}

Zhang, Houpu ^{[1
]}

Long, Zhengnan ^{[1
]}

Ge, Qiqing ^{[1
]}

Mei, Jiajia ^{[1
]}

Yu, Yunlong ^{[1
]}

Fang, Hua ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Agr & Biotechnol, Inst Pesticide & Environm Toxicol, Hangzhou 310058, Zhejiang, Peoples R China

来源：

CHEMOSPHERE | 2019年 / 228卷

基金：

美国国家科学基金会;

关键词：

Chlortetracycline; Ciprofloxacin; Biological function; Microbial community structure; Antibiotic-resistant bacteria; ANTIBIOTIC-RESISTANCE GENES; VETERINARY ANTIBIOTICS; TETRACYCLINE; BACTERIA; FATE; SULFAMONOMETHOXINE; SULFADIAZINE; DEGRADATION; DIVERSITY; RESISTOME;

D O I：

10.1016/j.chemosphere.2019.04.154

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The changes of enzyme activities, microbial community structure and function, and the diversity and resistance level of antibiotic-resistant bacteria (ARB) were studied in soil during ten repeated treatments with chlortetracycline (CTC) and/or ciprofloxacin (CIP) together with organic manure (OM) under laboratory conditions. The activities of neutral phosphatase (NPA) and catalase (CAT) displayed the suppression-recovery-stimulation trend in the OM&CTC treatment but the stimulation trend in the OM&CTC&CIP treatment. The NPA was stimulated but the CAT was little affected in the OM&CIP treatment. Soil microbial functional diversity displayed the suppression-recovery-stimulation trend in the OM&CTC and OM&CTC&CIP treatments and the stimulation-suppression trend in the OM&CIP treatment with the treatment frequency. Metagenomic analysis showed that the relative abundances of Actinobacteria and Firmicutes in the antibiotic treatment significantly increased by 0.5-235.6%, but that of Proreobacterta decreased by 0.2-27.3% compared to the control with the treatment frequency. Furthermore, the relative abundances of dominant bacterial genera including Streptomyces, Actinomadum, Mycobacterium, and Streptococcus in the antibiotic treatment significantly increased by 1.1-10433.3% compared to the control. Meanwhile, repeated antibiotic treatments induced a significant increase in the diversity and resistance level of ARB isolates, especially in the OM&CTC treatment. It is concluded that repeated treatments with CTC and/or CIP can alter enzyme activities, microbial community structure and function, and increase the diversity and resistance level of ARB isolates. (C) 2019 Elsevier Ltd. All rights reserved.

引用

页码：469 / 477

页数：9

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