4Pi-microscopy of the Golgi apparatus in live mammalian cells

被引：49

作者：

Egner, A

Verrier, S

Goroshkov, A

Söling, HD

Hell, SW ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NanoBiophoton, High Resolut Opt Microscopy Grp, D-37077 Gottingen, Germany

[2] Max Planck Inst Biophys Chem, Dept Neurobiol, D-37077 Gottingen, Germany

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2004年 / 147卷 / 01期

关键词：

D O I：

10.1016/j.jsb.2003.10.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the applicability of 4Pi-microscopy to live mammalian cells. Controlled interference of the counterpropagating wavefronts is possible despite the slight variations in cellular refractive index. Superresolved 3D-fluorescence imaging is exemplified with the first representation of the Golgi apparatus in a live cell at similar to100nm resolution. (C) 2003 Elsevier Inc. All rights reserved.

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页码：70 / 76

页数：7

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