Phenalenone-type phytoalexins mediate resistance of banana plants (Musa spp.) to the burrowing nematode Radopholus similis

被引：110

作者：

Hoelscher, Dirk ^{[1
,4
]}

Dhakshinamoorthy, Suganthagunthalam ^{[5
]}

Alexandrov, Theodore ^{[6
,7
,8
,9
]}

Becker, Michael ^{[10
]}

Bretschneider, Tom ^{[11
]}

Buerkert, Andreas ^{[4
]}

Crecelius, Anna C. ^{[12
]}

De Waele, Dirk ^{[5
,15
]}

Elsen, Annemie ^{[16
]}

Heckel, David G. ^{[3
]}

Heklau, Heike ^{[17
]}

Hertweck, Christian ^{[16
]}

Kai, Marco ^{[2
]}

Knop, Katrin ^{[12
]}

Krafft, Christoph ^{[18
]}

Maddula, Ravi K. ^{[2
]}

Matthaeus, Christian ^{[18
]}

Popp, Juergen ^{[13
,14
,18
]}

Schneider, Bernd ^{[1
]}

Schubert, Ulrich S. ^{[12
,19
]}

Sikora, Richard A. ^{[20
]}

Svatos, Ales ^{[2
]}

Swennen, Rony L. ^{[5
,21
,22
]}

机构：

[1] Max Planck Inst Chem Ecol, Nucl Magnet Resonance Res Grp, D-07745 Jena, Germany

[2] Max Planck Inst Chem Ecol, Mass Spectrometry Res Grp, D-07745 Jena, Germany

[3] Max Planck Inst Chem Ecol, Dept Entomol, D-07745 Jena, Germany

[4] Univ Kassel, Dept Organ Plant Prod & Agroecosyst Res Trop & Su, D-37213 Witzenhausen, Germany

[5] Univ Louvain, Dept Biosyst, Fac Biosci Engn, Lab Trop Crop Improvement,Div Crop Biotech, B-3001 Louvain, Belgium

[6] Univ Bremen, Dept Math, Ctr Ind Math, D-28359 Bremen, Germany

[7] Steinbeis Innovat Ctr Sci Comp Life Sci, D-28359 Bremen, Germany

[8] Sci Comp Life Sci SCiLS GmbH, D-28359 Bremen, Germany

[9] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA

[10] Bruker Daltonik GmbH, Dept Applicat Matrix Assisted Laser Desorpt Ioniz, D-28359 Bremen, Germany

[11] Leibniz Inst Nat Prod Res & Infect Biol, Dept Biomol Chem, D-07745 Jena, Germany

[12] Univ Jena, Jena Ctr Soft Matter, Lab Organ & Macromol Chem, D-07743 Jena, Germany

[13] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany

[14] Univ Jena, Abbe Ctr Photon, Dept Chem & Earth Sci, D-07743 Jena, Germany

[15] North West Univ, Dept Nematol, Unit Environm Sci & Management, ZA-2520 Potchefstroom, South Africa

[16] Soil Serv Belgium, Dept Res & Dev, B-3001 Leuven Heverlee, Belgium

[17] Univ Halle Wittenberg, Dept Geobot, D-06108 Halle, Germany

[18] Inst Photon Technol, Workgrp Spectroscopy Imaging, D-07745 Jena, Germany

[19] Dutch Polymer Inst, NK-5612 AB Eindhoven, Netherlands

[20] Univ Bonn, Inst Crop Sci & Resource Conservat Phytomed, D-53115 Bonn, Germany

[21] Bioversity Int, B-3001 Louvain, Belgium

[22] Int Inst Trop Agr, Croydon CR9 3EE, England

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2014年 / 111卷 / 01期

基金：

欧盟第七框架计划;

关键词：

plant protection; induced plant defense; matrix-free LDI-MSI; CAENORHABDITIS-ELEGANS; ACUMINATA; ROOTS; INFECTION; STRATEGIES; DISEASE; GENOME; AAA;

D O I：

10.1073/pnas.1314168110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The global yield of bananas-one of the most important food crops-is severely hampered by parasites, such as nematodes, which cause yield losses up to 75%. Plant-nematode interactions of two banana cultivars differing in susceptibility to Radopholus similis were investigated by combining the conventional and spatially resolved analytical techniques H-1 NMR spectroscopy, matrix-free UV-laser desorption/ionization mass spectrometric imaging, and Raman microspectroscopy. This innovative combination of analytical techniques was applied to isolate, identify, and locate the banana-specific type of phytoalexins, phenylphenalenones, in the R. similis-caused lesions of the plants. The striking antinematode activity of the phenylphenalenone anigorufone, its ingestion by the nematode, and its subsequent localization in lipid droplets within the nematode is reported. The importance of varying local concentrations of these specialized metabolites in infected plant tissues, their involvement in the plant's defense system, and derived strategies for improving banana resistance are highlighted.

引用

页码：105 / 110

页数：6

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