Bricks and blueprints: methods and standards for DNA assembly

被引：189

作者：

Casini, Arturo ^{[1
]}

Storch, Marko ^{[1
]}

Baldwin, Geoffrey S. ^{[1
]}

Ellis, Tom ^{[2
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Life Sci, Ctr Synthet Biol & Innovat, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Bioengn, Ctr Synthet Biol & Innovat, London SW7 2AZ, England

来源：

NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2015年 / 16卷 / 09期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

SYNTHETIC BIOLOGY; IN-VITRO; CHEMICAL-SYNTHESIS; CLONING; CONSTRUCTION; DESIGN; TECHNOLOGIES; ALGORITHMS; PLATFORM; PLASMID;

D O I：

10.1038/nrm4014

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

DNA assembly is a key part of constructing gene expression systems and even whole chromosomes. In the past decade, a plethora of powerful new DNA assembly methods - including Gibson Assembly, Golden Gate and ligase cycling reaction (LCR) - have been developed. In this Innovation article, we discuss these methods as well as standards such as the modular cloning (MoClo) system, GoldenBraid, modular overlap-directed assembly with linkers (MODAL) and PaperClip, which have been developed to facilitate a streamlined assembly workflow, to aid the exchange of material between research groups and to create modular reusable DNA parts.

引用

页码：568 / 576

页数：9

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