Brucella abortus vaccine strain RB51 produces low levels of M-like O-antigen

被引：26

作者：

Cloeckaert, A ^{[1
]}

Zygmunt, MS ^{[1
]}

Guilloteau, LA ^{[1
]}

机构：

[1] INRA, Stn Pathol Aviaire & Parasitol, Pathol Infect & Immunol Lab, F-37380 Nouzilly, France

来源：

VACCINE | 2002年 / 20卷 / 13-14期

关键词：

Brucella abortus; vaccine; RB51; O-chain;

D O I：

10.1016/S0264-410X(02)00035-X

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Brucella abortits RB51 is a rough (R) stable vaccine strain used in cattle and is believed to be devoid of O-side chain. We analyzed by use of a panel of monoclonal antibodies (MAbs) directed against seven previously defined O-polysaccharide (O-PS) epitopes the O-chain expression in strain RB51. Two MAbs specific for the C/Y (A = M) and C (M > A) epitopes showed low bindings in ELISA to strain RB51. O-chain expression was further confirmed by Western blot after SDS-PAGE of strain RB51. In particular, the MAb of C (M > A) specificity, showing preferential binding to M-dominant smooth (S) Brucella strains, revealed in strain RB51 a typical smooth-lipopolysaccharide (S-LPS) pattern which resembled that of M-dominant S-LPS. Thus, the results clearly show that strain RB51 produces low levels of M-like O-antigen. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1820 / 1822

页数：3

共 12 条

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INFECTION AND IMMUNITY, 2000, 68 (07) :3927-3932

[12] DNA polymorphism at the omp-31 locus of Brucella spp.: evidence for a large deletion in Brucella abortus, and other species-specific markers [J].

Vizcaino, N ;

Verger, JM ;

Grayon, M ;

Zygmunt, MS ;

Cloeckaert, A .

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