Brucella abortus vaccine strain RB51 produces low levels of M-like O-antigen

被引：26
作者：
Cloeckaert, A [1 ]
Zygmunt, MS [1 ]
Guilloteau, LA [1 ]
机构：
[1] INRA, Stn Pathol Aviaire & Parasitol, Pathol Infect & Immunol Lab, F-37380 Nouzilly, France
来源：
VACCINE | 2002年 / 20卷 / 13-14期
关键词：
Brucella abortus; vaccine; RB51; O-chain;
D O I：
10.1016/S0264-410X(02)00035-X
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Brucella abortits RB51 is a rough (R) stable vaccine strain used in cattle and is believed to be devoid of O-side chain. We analyzed by use of a panel of monoclonal antibodies (MAbs) directed against seven previously defined O-polysaccharide (O-PS) epitopes the O-chain expression in strain RB51. Two MAbs specific for the C/Y (A = M) and C (M > A) epitopes showed low bindings in ELISA to strain RB51. O-chain expression was further confirmed by Western blot after SDS-PAGE of strain RB51. In particular, the MAb of C (M > A) specificity, showing preferential binding to M-dominant smooth (S) Brucella strains, revealed in strain RB51 a typical smooth-lipopolysaccharide (S-LPS) pattern which resembled that of M-dominant S-LPS. Thus, the results clearly show that strain RB51 produces low levels of M-like O-antigen. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：1820 / 1822
页数：3
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