Synergistic activity of carbosilane dendrimers in combination with maraviroc against HIV in vitro

被引：25

作者：

Vacas Cordoba, Enrique ^{[1
,2
,4
]}

Arnaiz, Eduardo ^{[3
,4
]}

De La Mata, Francisco J. ^{[3
,4
]}

Gomez, Rafael ^{[4
]}

Leal, Manuel ^{[5
]}

Pion, Marjorie ^{[1
,2
,4
]}

Angeles Munoz-Fernandez, Ma ^{[1
,2
,4
]}

机构：

[1] Hosp Gen Univ Gregorio Maranon, Lab InmunoBiol Mol, CIBER BBN, Alcala De Henares, Spain

[2] Univ Alcala, Inst Invest Sanit Gregorio Maranon, Alcala De Henares, Spain

[3] Univ Alcala, Dept Inorgan Chem, Alcala De Henares, Spain

[4] CIBER BNN, Ctr Invest Biomed Red Bioingn Biomat & Nanomed, Madrid, Spain

[5] Univ Seville, Virgen Rocio Univ Hosp, CSIC,Clin Unit Infect Dis Microbiol & Prevent Med, Inst Biomed Seville IBiS,Lab Immunovirol, Seville, Spain

来源：

AIDS | 2013年 / 27卷 / 13期

关键词：

carbosilane dendrimer; HIV; maraviroc; microbicides; synergy; TOPICAL MICROBICIDES; PREVENTION; INHIBITORS; TENOFOVIR; DRUGS;

D O I：

10.1097/QAD.0b013e328361fa4a

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Objective:Dendrimers have been shown as effective and safe anti-HIV compounds with great potential as topical microbicides. Currently, the most advanced microbicides are based on antiretrovirals. However, nowadays none of them has shown any success in clinical trials.Design:Antiviral activity and combinatorial profile of different combinations between CCR5 co-receptor antagonist, maraviroc (MRV), and polyanionic carbosilane dendrimers against HIV-1 strains were evaluated.Methods:Cellular in-vitro models were used to evaluate the antiviral action of combinations of carbosilane dendrimers/MRV against CCR5 and dual tropic viral strains in TZM.bl cells and peripheral blood mononuclear cells. Combinatorial analysis was performed using Calcusyn software.Results:In the majority of combinations tested, dendrimers showed synergistic profile with MRV against CCR5 and dual tropic HIV-1.Conclusion:The evaluated two-drug combinations increase their antiviral potency supporting further clinical investigations to develop combinatorial formulated topical microbicides to fight against the worldwide HIV spread.

引用

页码：2053 / 2058

页数：6

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