Automated Extraction of Plant Viral RNA Using the Nucleic Acid Instrument Based on Magnetic Nanobeads

被引：0

作者：

Dou, Yongjie ^{[1
,2
]}

Zhao, Xiaoli ^{[3
]}

Xu, Jiansheng ^{[1
]}

Deng, Congliang ^{[3
]}

Nie, Leng ^{[1
]}

Yu, Yang ^{[1
]}

Wang, Ming ^{[1
]}

Song, Tao ^{[1
]}

机构：

[1] Chinese Acad Sci, Beijing Key Lab Bioelectromagnetism, Inst Elect Engn, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100190, Peoples R China

[3] Plant Lab Beijing Inspect & Quarantine Ctr, Beijing 101300, Peoples R China

来源：

SENSORS, MEASUREMENT AND INTELLIGENT MATERIALS, PTS 1-4 | 2013年 / 303-306卷

关键词：

Magnetic Nanobeads; Nucleic acid extraction; Magnetic bead separation; Plant viral RNA; C VIRUS-RNA; DNA; SEPARATION; PURIFICATION; PARTICLES; TECHNOLOGY; BEADS;

D O I：

10.4028/www.scientific.net/AMM.303-306.646

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In this paper, an automated nucleic acid extraction instrument based on magnetic nanobeads is described. Also, a prototype is designed and constructed. Some plant viral RNA extraction experiments were done using the platform. And the experimental results demonstrated that the instrument could be successfully used for extraction of plant viral RNA, and the extracted RNA was successfully used in an automated PCR assay for the detection of plant virus. The Ct values of CGMMV RNA, LSV RNA and ArMV RNA at the initial concentration were 12.51, 17.81 and 23.48.

引用

页码：646 / +

页数：2

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