Automated Extraction of Plant Viral RNA Using the Nucleic Acid Instrument Based on Magnetic Nanobeads

被引：0

作者：

Dou, Yongjie ^{[1
,2
]}

Zhao, Xiaoli ^{[3
]}

Xu, Jiansheng ^{[1
]}

Deng, Congliang ^{[3
]}

Nie, Leng ^{[1
]}

Yu, Yang ^{[1
]}

Wang, Ming ^{[1
]}

Song, Tao ^{[1
]}

机构：

[1] Chinese Acad Sci, Beijing Key Lab Bioelectromagnetism, Inst Elect Engn, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100190, Peoples R China

[3] Plant Lab Beijing Inspect & Quarantine Ctr, Beijing 101300, Peoples R China

来源：

SENSORS, MEASUREMENT AND INTELLIGENT MATERIALS, PTS 1-4 | 2013年 / 303-306卷

关键词：

Magnetic Nanobeads; Nucleic acid extraction; Magnetic bead separation; Plant viral RNA; C VIRUS-RNA; DNA; SEPARATION; PURIFICATION; PARTICLES; TECHNOLOGY; BEADS;

D O I：

10.4028/www.scientific.net/AMM.303-306.646

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In this paper, an automated nucleic acid extraction instrument based on magnetic nanobeads is described. Also, a prototype is designed and constructed. Some plant viral RNA extraction experiments were done using the platform. And the experimental results demonstrated that the instrument could be successfully used for extraction of plant viral RNA, and the extracted RNA was successfully used in an automated PCR assay for the detection of plant virus. The Ct values of CGMMV RNA, LSV RNA and ArMV RNA at the initial concentration were 12.51, 17.81 and 23.48.

引用

页码：646 / +

页数：2

共 50 条

[31] A Comparison of Three Automated Nucleic Acid Extraction Systems for Human Stool Samples
Kwa, Wit Thun
Sim, Choon Kiat
Low, Adrian
Lee, Jonathan Wei Jie
MICROORGANISMS, 2024, 12 (12)
[32] Development of a Nucleic Acid Extraction Procedure for Simultaneous Recovery of DNA and RNA from Diverse Microbes in Water
Hill, Vincent R.
Narayanan, Jothikumar
Gallen, Rachel R.
Ferdinand, Karen L.
Cromeans, Theresa
Vinje, Jan
PATHOGENS, 2015, 4 (02) : 335 - 354
[33] RNA based viral silencing suppression in plant pararetroviruses
Hohn, Thomas
FRONTIERS IN PLANT SCIENCE, 2015, 6
[34] Rapid extraction of genomic DNA from saliva for HLA typing on microarray based on magnetic nanobeads
Xie, X
Zhang, X
Yu, BB
Gao, HF
Zhang, H
Fei, WY
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2004, 280 (2-3) : 164 - 168
[35] Preparation of molecularly imprinted polymers based on magnetic nanoparticles for the selective extraction of protocatechuic acid from plant extracts
Xie, Xiaoyu
Wei, Fen
Chen, Liang
Wang, Sicen
JOURNAL OF SEPARATION SCIENCE, 2015, 38 (06) : 1046 - 1052
[36] Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents
Haile, Simon
Nikiforuk, Aidan M.
Pandoh, Pawan K.
Twa, David D. W.
Smailus, Duane E.
Nguyen, Jason
Pleasance, Stephen
Wong, Angus
Zhao, Yongjun
Eisler, Diane
Moksa, Michelle
Cao, Qi
Wong, Marcus
Su, Edmund
Krzywinski, Martin
Nelson, Jessica
Mungall, Andrew J.
Tsang, Frankie
Prentice, Leah M.
Jassem, Agatha
Manges, Amee R.
Jones, Steven J. M.
Coope, Robin J.
Prystajecky, Natalie
Marra, Marco A.
Krajden, Mel
Hirst, Martin
JOURNAL OF VIROLOGICAL METHODS, 2022, 299
[37] Comparative study on three viral enrichment approaches based on RNA extraction for plant virus/viroid detection using high-throughput sequencing
Gaafar, Yahya Zakaria Abdou
Ziebell, Heiko
PLOS ONE, 2020, 15 (08):
[38] A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: An example using Bluetongue virus
Vandemeulebroucke, Elise
De Clercq, Kris
Van der Stede, Yves
Vandenbussche, Frank
JOURNAL OF VIROLOGICAL METHODS, 2010, 165 (01) : 76 - 82
[39] Comparison of six nucleic acid extraction methods for detection of viral DNA or RNA sequences in four different non-serum specimen types
Kok, T
Wati, S
Bayly, B
Devonshire-Gill, D
Higgins, G
JOURNAL OF CLINICAL VIROLOGY, 2000, 16 (01) : 59 - 63
[40] Integrated DNA and RNA extraction using magnetic beads from viral pathogens causing acute respiratory infections
He, Hui
Li, Rongqun
Chen, Yi
Pan, Ping
Tong, Wenjuan
Dong, Xueyan
Chen, Yueming
Yu, Daojun
SCIENTIFIC REPORTS, 2017, 7

← 1 2 3 4 5 →