Automated Extraction of Plant Viral RNA Using the Nucleic Acid Instrument Based on Magnetic Nanobeads

被引：0

作者：

Dou, Yongjie ^{[1
,2
]}

Zhao, Xiaoli ^{[3
]}

Xu, Jiansheng ^{[1
]}

Deng, Congliang ^{[3
]}

Nie, Leng ^{[1
]}

Yu, Yang ^{[1
]}

Wang, Ming ^{[1
]}

Song, Tao ^{[1
]}

机构：

[1] Chinese Acad Sci, Beijing Key Lab Bioelectromagnetism, Inst Elect Engn, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100190, Peoples R China

[3] Plant Lab Beijing Inspect & Quarantine Ctr, Beijing 101300, Peoples R China

来源：

SENSORS, MEASUREMENT AND INTELLIGENT MATERIALS, PTS 1-4 | 2013年 / 303-306卷

关键词：

Magnetic Nanobeads; Nucleic acid extraction; Magnetic bead separation; Plant viral RNA; C VIRUS-RNA; DNA; SEPARATION; PURIFICATION; PARTICLES; TECHNOLOGY; BEADS;

D O I：

10.4028/www.scientific.net/AMM.303-306.646

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

In this paper, an automated nucleic acid extraction instrument based on magnetic nanobeads is described. Also, a prototype is designed and constructed. Some plant viral RNA extraction experiments were done using the platform. And the experimental results demonstrated that the instrument could be successfully used for extraction of plant viral RNA, and the extracted RNA was successfully used in an automated PCR assay for the detection of plant virus. The Ct values of CGMMV RNA, LSV RNA and ArMV RNA at the initial concentration were 12.51, 17.81 and 23.48.

引用

页码：646 / +

页数：2

共 50 条

[21] Automated nucleic acid isolation in viral molecular diagnostics: evaluation of the QIAsymphony SP
Parham, N. J.
Parmar, S. A.
Kumar, N.
Aliyu, S.
Curran, M. D.
Zhang, H.
BRITISH JOURNAL OF BIOMEDICAL SCIENCE, 2012, 69 (01) : 18 - 25
[22] Rapid Nucleic Acid Extraction and Purification Using a Miniature Ultrasonic Technique
Branch, Darren W.
Vreeland, Erika C.
McClain, Jamie L.
Murton, Jaclyn K.
James, Conrad D.
Achyuthan, Komandoor E.
MICROMACHINES, 2017, 8 (07):
[23] Magnetic bead technology for viral RNA extraction from serum in blood bank screening
Ambrozio Albertoni, Guilherme
Arnoni, Carine Prisco
Barboza Araujo, Patricia Regina
Andrade, Sheila Siqueira
Carvalho, Fabricio Oliveira
Batista Castello Girao, Manoel Joao
Schor, Nestor
Barreto, Jose Augusto
BRAZILIAN JOURNAL OF INFECTIOUS DISEASES, 2011, 15 (06) : 547 - 552
[24] A novel miniaturized biofilter based on silicon micropillars for nucleic acid extraction
Petralia, Salvatore
Sciuto, Emanuele Luigi
Conoci, Sabrina
ANALYST, 2017, 142 (01) : 140 - 146
[25] Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens
Mengelle, C.
Mansuy, J. -M.
Sandres-Saune, K.
Barthe, C.
Boineau, J.
Izopet, J.
JOURNAL OF MEDICAL VIROLOGY, 2012, 84 (06) : 906 - 911
[26] Comparison of Nucleic Acid Extraction Methods for a Viral Metagenomics Analysis of Respiratory Viruses
Sabatier, Marina
Bal, Antonin
Destras, Gregory
Regue, Hadrien
Queromes, Gregory
Cheynet, Valerie
Lina, Bruno
Bardel, Claire
Brengel-Pesce, Karen
Navratil, Vincent
Josset, Laurence
MICROORGANISMS, 2020, 8 (10) : 1 - 18
[27] Optimalization of deoxyribonucleic acid extraction using various types of magnetic particles
Konecna, Jana
Romanovska, Denisa
Horak, Daniel
Trachtova, Stepanka
CHEMICAL PAPERS, 2019, 73 (05): : 1247 - 1255
[28] Parallel RNA extraction using magnetic beads and a droplet array
Shi, Xu
Chen, Chun-Hong
Gao, Weimin
Chao, Shih-hui
Meldrum, Deirdre R.
LAB ON A CHIP, 2015, 15 (04) : 1059 - 1065
[29] Comparison of Manual and Automated Nucleic Acid Extraction Methods in Virus Transport Medium
Chen, Si-Yu
Chou, Yung-Che
Huang, Jian-Wei
Er, Tze-Kiong
CLINICAL LABORATORY, 2021, 67 (12) : 2753 - 2755
[30] Automated microfluidic system for rapid, multiplexed nucleic acid extraction and detection of pathogens
Cheng, Lin
Wang, Ru
Zou, Changhua
Yan, Jing
SENSORS AND ACTUATORS B-CHEMICAL, 2025, 426

← 1 2 3 4 5 →