Titanium dioxide nanoparticles inhibit proliferation and induce morphological changes and apoptosis in glial cells

被引：75

作者：

Gissela Marquez-Ramirez, Sandra ^{[1
,2
]}

Laura Delgado-Buenrostro, Norma ^{[3
]}

Irasema Chirino, Yolanda ^{[3
]}

Gutierrez Iglesias, Gisela ^{[2
]}

Lopez-Marure, Rebeca ^{[1
]}

机构：

[1] Inst Nacl Cardiol Ignacio Chavez, Dept Biol Celular, Mexico City 14080, DF, Mexico

[2] Inst Politecn Nacl, Escuela Super Med, Dept Posgrad, Mexico City 07738, DF, Mexico

[3] Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City, DF, Mexico

来源：

TOXICOLOGY | 2012年 / 302卷 / 2-3期

关键词：

Nanoparticles; Titanium dioxide; Glial cells; Proliferation; Apoptosis; CENTRAL-NERVOUS-SYSTEM; TIO2; NANOPARTICLES; ULTRAFINE PARTICLES; BRAIN MICROGLIA; TOXICITY; TRANSLOCATION; CYTOTOXICITY; MANGANESE; EXPOSURE; OXIDE;

D O I：

10.1016/j.tox.2012.09.005

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Titanium dioxide nanoparticles (TiO2 NPs) are widely used in the chemical, electrical and electronic industries. TiO2 NPs can enter directly into the brain through the olfactory bulb and be deposited in the hippocampus region. We determined the effect of TiO2 NPs on rat and human glial cells, C6 and U373, respectively. We evaluated proliferation by crystal violet staining, internalization of TiO2 NPs, and cellular morphology by TEM analysis, as well as F-actin distribution by immunostaining and cell death by detecting active caspase-3 and DNA fragmentation. TiO2 NPs inhibited proliferation and induced morphological changes that were related with a decrease in immuno-location of F-actin fibers. TiO2 NPs were internalized and formation of vesicles was observed. TiO2 NPs induced apoptosis after 96h of treatment. Hence, TiO2 NPs had a cytotoxic effect on glial cells, suggesting that exposure to TiO2 NPs could cause brain injury and be hazardous to health. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：146 / 156

页数：11

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