Determination of urinary triclosan by stir bar sorptive extraction and thermal desorption-gas chromatography-mass spectrometry

被引：32

作者：

Kawaguchi, Migaku ^{[1
]}

Ito, Rie ^{[1
]}

Honda, Hidehiro ^{[1
]}

Endo, Naoyuki ^{[1
]}

Okanouchi, Noriya ^{[1
]}

Saito, Koichi ^{[1
]}

Seto, Yasuo ^{[2
]}

Nakazawa, Hiroyuki ^{[1
]}

机构：

[1] Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, Tokyo 1428501, Japan

[2] Natl Res Inst Police Sci, Kashiwa, Chiba 2770882, Japan

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 875卷 / 02期

关键词：

Triclosan; Urine sample; Stir bar sorptive extraction (SBSE); Thermal desorption (TD); Gas chromatography-mass spectrometry (GC-MS);

D O I：

10.1016/j.jchromb.2008.10.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed an analytical method for the determination of urinary 5-cliloro-2-(2,4-dichlorophenoxy)phenol (triclosan), which utilizes stir bar sorptive extraction (SBSE) and thermal desorption (TD)-gas chromatography-mass spectrometry (GC-MS). Human urine sample is deconjugated by treatment with P-glucuronidase and sulfatase. A stir bar coated with polydimethylsiloxane (PDMS) is added to the urine sample in a vial and the sample is stirred for 60min at room temperature (25 degrees C). Then, the PDMS stir bar is subjected to TD-GC-MS. The detection limit of triclosan is 0.05 ngmL(-1). The method shows linearity over the calibration range (0.1-10ng mL(-1)) and the correlation coefficient (r) is higher than 0.993 for triclosan standard solution. The average recoveries of triclosan in human urine sample are 102.8-113.1% (RSD: 2.4-6.7%). This simple, sensitive, and selective analytical method may be used in the determination of trace amounts of triclosan in human urine samples. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：577 / 580

页数：4

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