Tracking X-ray microscopy for alveolar dynamics in live intact mice

被引：11

作者：

Chang, Soeun ^{[1
,2
]}

Kwon, Namseop ^{[1
,3
]}

Weon, Byung Mook ^{[1
,2
]}

Kim, Jinkyung ^{[1
,3
]}

Rhee, Chin Kook ^{[4
]}

Choi, Han Sung ^{[5
]}

Kohmura, Yoshiki ^{[6
]}

Yamamoto, Masaki ^{[6
]}

Ishikawa, Tetsuya ^{[6
]}

Je, Jung Ho ^{[1
,2
,6
]}

机构：

[1] Pohang Univ Sci & Technol, Xray Imaging Ctr, Pohang 790784, South Korea

[2] Pohang Univ Sci & Technol, Dept Mat Sci & Engn, Pohang 790784, South Korea

[3] Pohang Univ Sci & Technol, Sch Interdisciplinary Biosci & Bioengn, Pohang 790784, South Korea

[4] Catholic Univ Korea, Dept Internal Med, Div Pulm & Crit Care Med, Seoul St Marys Hosp, Seoul 137701, South Korea

[5] Kyung Hee Univ Hosp, Dept Emergency Med, Seoul 130872, South Korea

[6] RIKEN SPring 8 Ctr, Sayo, Hyogo 6795198, Japan

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

关键词：

D O I：

10.1038/srep01304

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Here we report a tracking X-ray microscopy (TrXM) as a novel methodology by using upper right lung apices alveoli in live intact mice. By enabling tracking of individual alveolar movements during respiration, TrXM identifies alveolar dynamics: individual alveoli in the upper lung apices show a small size increment as 4.9 +/- 0.4% (mean +/- s.e.m.) during respiration while their shapes look almost invariant. TrXM analysis in alveolar dynamics would be significant for better understanding of alveolar-based diseases, for instance, ventilator induced lung injury (VILI) in acute respiratory distress syndrome (ARDS).

引用

页数：5

共 14 条

[1] Ventilation with lower tidal volumes as compared with traditional tidal volumes for acute lung injury and the acute respiratory distress syndrome.
Brower, RG
Matthay, MA
Morris, A
Schoenfeld, D
Thompson, BT
Wheeler, A
Wiedemann, HP
Arroliga, AC
Fisher, CJ
Komara, JJ
Perez-Trepichio, P
Parsons, PE
Wolkin, R
Welsh, C
Fulkerson, WJ
MacIntyre, N
Mallatratt, L
Sebastian, M
McConnell, R
Wilcox, C
Govert, J
Thompson, D
Clemmer, T
Davis, R
Orme, J
Weaver, L
Grissom, C
Eskelson, M
Young, M
Gooder, V
McBride, K
Lawton, C
d'Hulst, J
Peerless, JR
Smith, C
Brownlee, J
Pluss, W
Kallet, R
Luce, JM
Gottlieb, J
Elmer, M
Girod, A
Park, P
Daniel, B
Gropper, M
Abraham, E
Piedalue, F
Glodowski, J
Lockrem, J
McIntyre, R
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 2000, 342 (18) : 1301 - 1308
[2] Carney David, 2005, Crit Care Med, V33, pS122, DOI 10.1097/01.CCM.0000155928.95341.BC
[3] Cotes J.E., 2009, Lung function: physiology, measurement and application in medicine
[4] Synchrotron-based dynamic computed tomography of tissue motion for regional lung function measurement
Dubsky, Stephen
Hooper, Stuart B.
Siu, Karen K. W.
Fouras, Andreas
[J]. JOURNAL OF THE ROYAL SOCIETY INTERFACE, 2012, 9 (74) : 2213 - 2224
[5] Gatto Louis A, 2004, Respir Care, V49, P1045
[6] Flow in a terminal alveolar sac model with expanding walls using computational fluid dynamics
Harding, Edward M., Jr.
Robinson, Risa J.
[J]. INHALATION TOXICOLOGY, 2010, 22 (08) : 669 - 678
[7] Synchrotron microangiography with no contrast agent
Hwu, Y
Tsai, WL
Je, JH
Seol, SK
Kim, B
Groso, A
Margaritondo, G
Lee, KH
Seong, JK
[J]. PHYSICS IN MEDICINE AND BIOLOGY, 2004, 49 (04) : 501 - 508
[8] Stabilized imaging of immune surveillance in the mouse lung
Looney, Mark R.
Thornton, Emily E.
Sen, Debasish
Lamm, Wayne J.
Glenny, Robb W.
Krummel, Matthew F.
[J]. NATURE METHODS, 2011, 8 (01) : 91 - U133
[9] Virtual four-dimensional imaging of lung parenchyma by optical coherence tomography in mice
Meissner, Sven
Tabuchi, Arata
Mertens, Michael
Kuebler, Wolfgang M.
Koch, Edmund
[J]. JOURNAL OF BIOMEDICAL OPTICS, 2010, 15 (03)
[10] Alveolar dynamics in acute lung injury: Heterogeneous distension rather than cyclic opening and collapse
Mertens, Michael
Tabuchi, Arata
Meissner, Sven
Krueger, Alexander
Schirrmann, Kerstin
Kertzscher, Ulrich
Pries, Axel R.
Slutsky, Arthur S.
Koch, Edmund
Kuebler, Wolfgang M.
[J]. CRITICAL CARE MEDICINE, 2009, 37 (09) : 2604 - 2611

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