Combinatorial surrobody libraries

被引：14

作者：

Xu, Li ^{[1
]}

Yee, Helena ^{[1
]}

Chan, Christina ^{[1
]}

Kashyap, Arun K. ^{[1
]}

Horowitz, Lawrence ^{[1
]}

Horowitz, Michael ^{[1
]}

Bhatt, Ramesh R. ^{[1
]}

Lerner, Richard A. ^{[2
]}

机构：

[1] Sea Lane Biotechnol, Menlo Pk, CA 94025 USA

[2] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 31期

关键词：

antibody engineering; combinatorial antibody libraries; surrogate light chain;

D O I：

10.1073/pnas.0805293105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A unique type of combinatorial protein libraries has been constructed. These libraries are based on the pre-B cell receptor (pre-BCR). The pre-BCR is a protein that is produced during normal development of the antibody repertoire. Unlike that of canonical antibodies, the pre-BCR subunit is a trimer that is composed of an antibody heavy chain paired with two surrogate light chain (SLC) components. Combinatorial libraries based on these pre-BCR proteins in which diverse heavy chains are paired with a fixed SLC were expressed in mammalian, Escherichia coli, and phagemid systems. These libraries contain members that have nanomolar affinity for antigen. We term this type of antigen-binding protein a "surrobody" to distinguish it from the canonical antibody molecule.

引用

页码：10756 / 10761

页数：6

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