Multiphoton Microscopy for Ophthalmic Imaging

被引：50

作者：

Gibson, Emily A. ^{[2
]}

Masihzadeh, Omid ^{[1
,3
]}

Lei, Tim C. ^{[3
]}

Ammar, David A. ^{[1
]}

Kahook, Malik Y. ^{[1
]}

机构：

[1] Univ Colorado, Sch Med, Dept Ophthalmol, Aurora, CO 80045 USA

[2] Univ Colorado, Dept Bioengn, Denver, CO 80045 USA

[3] Univ Colorado, Dept Elect Engn, Denver, CO 80217 USA

来源：

JOURNAL OF OPHTHALMOLOGY | 2011年 / 2011卷

关键词：

SUBCELLULAR SPATIAL-RESOLUTION; IN-VIVO; 2-PHOTON MICROSCOPE; ADAPTIVE-OPTICS; FLUORESCENCE; AUTOFLUORESCENCE; TISSUES; CELLS; FIBER; SENSITIVITY;

D O I：

10.1155/2011/870879

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

We review multiphoton microscopy (MPM) including two-photon autofluorescence (2PAF), second harmonic generation (SHG), third harmonic generation (THG), fluorescence lifetime (FLIM), and coherent anti-Stokes Raman Scattering (CARS) with relevance to clinical applications in ophthalmology. The different imaging modalities are discussed highlighting the particular strength that each has for functional tissue imaging. MPM is compared with current clinical ophthalmological imaging techniques such as reflectance confocal microscopy, optical coherence tomography, and fluorescence imaging. In addition, we discuss the future prospects for MPM in disease detection and clinical monitoring of disease progression, understanding fundamental disease mechanisms, and real-time monitoring of drug delivery.

引用

页数：11

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