Antioxidant activity and cytotoxicity against cholangiocarcinoma of carbazoles and coumarins from Clausena harmandiana

被引：35

作者：

Songsiang, Uraiwan ^{[1
,2
]}

Thongthoom, Tula ^{[1
,2
]}

Zeekpudsa, Pornsilp ^{[3
]}

Kukongviriyapan, Veerapol ^{[3
]}

Boonyarat, Chantana ^{[4
]}

Wangboonskul, Jinda ^{[4
]}

Yenjai, Chavi ^{[1
,2
]}

机构：

[1] Khon Kaen Univ, Fac Sci, Dept Chem, Nat Prod Res Unit, Khon Kaen 40002, Thailand

[2] Khon Kaen Univ, Fac Sci, Ctr Innovat Chem, Khon Kaen 40002, Thailand

[3] Khon Kaen Univ, Fac Med, Dept Pharmacol, Khon Kaen 40002, Thailand

[4] Khon Kaen Univ, Fac Pharmaceut Sci, Khon Kaen 40002, Thailand

来源：

SCIENCEASIA | 2012年 / 38卷 / 01期

关键词：

lipid peroxidation; antioxidants; free radicals; natural products; drug development; 1ST TOTAL-SYNTHESIS; PALLADIUM-CATALYZED APPROACH; ORGANIC-SYNTHESIS; CONSTITUENTS; ALKALOIDS; EXCAVATA;

D O I：

10.2306/scienceasia1513-1874.2012.38.075

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nine carbazoles and three coumarins were isolated from C. harmandiana. Crude extracts and all isolates were evaluated for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and lipid peroxidation assay and cytotoxicity against cholangiocarcinoma, KKU-OCA17 and KKU-214 cell lines. 7-Hydroxyheptaphylline and nordentatin showed strong antioxidant activity on lipid peroxidation with IC50 values of 2.95 and 2.90 mu M respectively, while the activity on a DPPH assay showed IC50 values of 56.82 and 29.3 mu M, respectively. The two compounds exhibited strong cytotoxicity against KKU-OCA17 (IC50=88.7 and 46.1 mu M, respectively) and KKU-214 (IC50=43.7 and 39.1 mu M, respectively) cell lines. The results show convincingly that these compounds may be the promising leads for the development of cytotoxic agents.

引用

页码：75 / 81

页数：7

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