Antibacterial Activity of Glutathione-Coated Silver Nanoparticles against Gram Positive and Gram Negative Bacteria

被引：261

作者：

Taglietti, Angelo ^{[1
]}

Fernandez, Yuri A. Diaz ^{[1
]}

Amato, Elvio ^{[1
]}

Cucca, Lucia ^{[1
]}

Dacarro, Giacomo ^{[2
]}

Grisoli, Pietro ^{[3
]}

Necchi, Vittorio ^{[4
]}

Pallavicini, Piersandro ^{[1
]}

Pasotti, Luca ^{[1
]}

Patrini, Maddalena ^{[2
]}

机构：

[1] Univ Pavia, Sez Chim Gen, Dipartimento Chim, I-27100 Pavia, Italy

[2] Univ Pavia, Dipartimento Fis A Volta, I-27100 Pavia, Italy

[3] Univ Pavia, Dipartimento Sci Farmaco, I-27100 Pavia, Italy

[4] Univ Pavia, Ctr Grandi Strumenti, I-27100 Pavia, Italy

来源：

LANGMUIR | 2012年 / 28卷 / 21期

关键词：

ESCHERICHIA-COLI; BIOMEDICAL APPLICATIONS; ELECTRON-MICROSCOPY; IMPLANT INFECTIONS; AG; CELLS; RESISTANCE; PARTICLES; TOXICITY; SHAPES;

D O I：

10.1021/la3003838

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the present paper, we study the mechanism of antibacterial activity of glutathione (GSH) coated silver nanoparticles (Ag NPs) on model Gram negative and Gram positive bacterial strains. Interference in bacterial cell replication is observed for both cellular strains when exposed to GSH stabilized colloidal silver in solution, and microbicidal activity was studied when GSH coated Ag NPs are (i) dispersed in colloidal suspensions or (ii) grafted on thiol-functionalized glass surfaces. The obtained results confirm that the effect of dispersed GSH capped Ag NPs (GSH Ag NPs) on Escherichia colt is more intense because it can be associated with the penetration of the colloid into the cytoplasm, with the subsequent local interaction of silver with cell components causing damages to the cells. Conversely, for Staphylococcus aureus, since the thick peptidoglycan layer of the cell wall prevents the penetration of the NPs inside the cytoplasm, the antimicrobial effect is limited and seems related to the interaction with the bacterial surfaces. Experiments on GSH Ag NPs grafted on glass allowed us to elucidate more precisely the antibacterial mechanism, showing that the action is reduced because of GSH coating and the limitation of the translational freedom of NPs.

引用

页码：8140 / 8148

页数：9