Coherent X-Ray Imaging of Collagen Fibril Distributions within Intact Tendons

被引：10

作者：

Berenguer, Felisa ^{[1
]}

Bean, Richard J. ^{[1
]}

Bozec, Laurent ^{[1
,2
]}

Vila-Comamala, Joan ^{[3
]}

Zhang, Fucai ^{[4
]}

Kewish, Cameron M. ^{[3
]}

Bunk, Oliver ^{[3
]}

Rodenburg, John M. ^{[4
,5
]}

Robinson, Ian K. ^{[1
,5
]}

机构：

[1] UCL, London Ctr Nanotechnol, London, England

[2] UCL, Biomat & Tissue Engn Eastman Dent Inst, London, England

[3] Paul Scherrer Inst, Villigen, Switzerland

[4] Univ Sheffield, Dept Elect & Elect Engn, Sheffield S1 3JD, S Yorkshire, England

[5] Res Complex Harwell, Didcot, Oxon, England

来源：

BIOPHYSICAL JOURNAL | 2014年 / 106卷 / 02期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

ATOMIC-FORCE MICROSCOPY; MECHANICAL-PROPERTIES; DIFFRACTION MICROSCOPY; CONNECTIVE TISSUES; I COLLAGEN; NANOSCALE; ALGORITHM; BONE; SIZE;

D O I：

10.1016/j.bpj.2013.12.016

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The characterization of the structure of highly hierarchical biosamples such as collagen-based tissues at the scale of tens of nanometers is essential to correlate the tissue structure with its growth processes. Coherent x-ray Bragg ptychography is an innovative imaging technique that gives high resolution images of the ordered parts of such samples. Herein, we report how we used this method to image the collagen fibrillar ultrastructure of intact rat tail tendons. The images show ordered fibrils extending over 10-20 mu m in length, with a quantifiable D-banding spacing variation of 0.2%. Occasional defects in the fibrils distribution have also been observed, likely indicating fibrillar fusion events.

引用

页码：459 / 466

页数：8

共 39 条

[1] Molecular mechanisms of ageing in connective tissues
Bailey, AJ
[J]. MECHANISMS OF AGEING AND DEVELOPMENT, 2001, 122 (07) : 735 - 755
[2] Bean R. J., 2012, THESIS U COLL LONDON
[3] Collagen fibrils: Nanoscale ropes
Bozec, Laurent
van der Heijden, Gert
Horton, Michael
[J]. BIOPHYSICAL JOURNAL, 2007, 92 (01) : 70 - 75
[4] Thermal Denaturation Studies of Collagen by Microthermal Analysis and Atomic Force Microscopy
Bozec, Laurent
Odlyha, Marianne
[J]. BIOPHYSICAL JOURNAL, 2011, 101 (01) : 228 - 236
[5] Nature designs tough collagen: Explaining the nanostructure of collagen fibrils
Buehler, Markus J.
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (33) : 12285 - 12290
[6] Engineering functional collagen scaffolds: Cyclical loading increases material strength and fibril aggregation
Cheema, Umber
Chuo, Cher-Bing
Sarathchandra, Padmini
Nazhat, Showan N.
Brown, Robert A.
[J]. ADVANCED FUNCTIONAL MATERIALS, 2007, 17 (14) : 2426 - 2431
[7] Assembly of type I collagen: fusion of fibril subunits and the influence of fibril diameter on mechanical properties
Christiansen, DL
Huang, EK
Silver, FH
[J]. MATRIX BIOLOGY, 2000, 19 (05) : 409 - 420
[8] Observing growth steps of collagen self-assembly by time-lapse high-resolution atomic force microscopy
Cisneros, David A.
Hung, Carlos
Franz, Clemens A.
Muller, Daniel J.
[J]. JOURNAL OF STRUCTURAL BIOLOGY, 2006, 154 (03) : 232 - 245
[9] Rat-tail tendon fiber SAXS high-order diffraction peaks recovered by a superbright laboratory source and a novel restoration algorithm
De Caro, Liberato
Altamura, Davide
Sibillano, Teresa
Siliqi, Dritan
Filograsso, Giovanni
Bunk, Oliver
Giannini, Cinzia
[J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 2013, 46 : 672 - 678
[10] Collagen imaged by Coherent X-ray Diffraction: towards a complementary tool to conventional scanning SAXS
de la Cuesta, Felisa Berenguer
Bean, Richard J.
McCallion, Catriona
Wallace, Kris
Bozec, Laurent
Hiller, Jen C.
Terrill, Nicholas J.
Robinson, Ian K.
[J]. XIV INTERNATIONAL CONFERENCE ON SMALL-ANGLE SCATTERING (SAS09), 2010, 247

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