Coherent X-Ray Imaging of Collagen Fibril Distributions within Intact Tendons

被引：10

作者：

Berenguer, Felisa ^{[1
]}

Bean, Richard J. ^{[1
]}

Bozec, Laurent ^{[1
,2
]}

Vila-Comamala, Joan ^{[3
]}

Zhang, Fucai ^{[4
]}

Kewish, Cameron M. ^{[3
]}

Bunk, Oliver ^{[3
]}

Rodenburg, John M. ^{[4
,5
]}

Robinson, Ian K. ^{[1
,5
]}

机构：

[1] UCL, London Ctr Nanotechnol, London, England

[2] UCL, Biomat & Tissue Engn Eastman Dent Inst, London, England

[3] Paul Scherrer Inst, Villigen, Switzerland

[4] Univ Sheffield, Dept Elect & Elect Engn, Sheffield S1 3JD, S Yorkshire, England

[5] Res Complex Harwell, Didcot, Oxon, England

来源：

BIOPHYSICAL JOURNAL | 2014年 / 106卷 / 02期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

ATOMIC-FORCE MICROSCOPY; MECHANICAL-PROPERTIES; DIFFRACTION MICROSCOPY; CONNECTIVE TISSUES; I COLLAGEN; NANOSCALE; ALGORITHM; BONE; SIZE;

D O I：

10.1016/j.bpj.2013.12.016

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The characterization of the structure of highly hierarchical biosamples such as collagen-based tissues at the scale of tens of nanometers is essential to correlate the tissue structure with its growth processes. Coherent x-ray Bragg ptychography is an innovative imaging technique that gives high resolution images of the ordered parts of such samples. Herein, we report how we used this method to image the collagen fibrillar ultrastructure of intact rat tail tendons. The images show ordered fibrils extending over 10-20 mu m in length, with a quantifiable D-banding spacing variation of 0.2%. Occasional defects in the fibrils distribution have also been observed, likely indicating fibrillar fusion events.

引用

页码：459 / 466

页数：8

共 39 条

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