Biosynthesis of thiamin thiazole: Determination of the regiochemistry of the S/O acyl shift by using 1,4-dideoxy-D-xylulose-5-phosphate

被引：7

作者：

Chatterjee, Abhishek ^{[1
]}

Han, Xuemei ^{[1
]}

McLafferty, Fred W. ^{[1
]}

Begley, Tadhg P. ^{[1
]}

机构：

[1] Cornell Univ, Dept Chem & Chem Biol, Ithaca, NY 14853 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 21期

关键词：

acyl shift; biosynthesis; enzyme catalysis; isotopic labeling; vitamins;

D O I：

10.1002/anie.200504614

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) ThiS and that: An alternative substrate for thiazole synthase, namely 1,4-dideoxy-D-xylulose-5-phosphate, was synthesized and used as a mechanistic probe to determine the regiochemistry of the acyl-shift reaction that occurs as an intermediate step in the biosynthesis of thiamin thiazole in B. subtilis (see scheme; ThiS protein (blue); ThiG protein (red); OP=phosphate group). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3507 / 3510

页数：4

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