Metallated porphyrin-doped conjugated polymer nanoparticles for efficient photodynamic therapy of brain and colorectal tumor cells

被引：39

作者：

Exequiel Ibarra, Luis ^{[1
,2
]}

Valeria Porcal, Gabriela ^{[1
,3
,4
]}

Paola Macor, Lorena ^{[1
,3
,4
]}

Andre Ponzio, Rodrigo ^{[1
,4
,5
]}

Martin Spada, Ramiro ^{[1
,3
,4
]}

Lorente, Carolina ^{[6
,7
]}

Alberto Chesta, Carlos ^{[1
,3
,4
]}

Alicia Rivarola, Viviana ^{[1
,2
]}

Emiliano Palacios, Rodrigo ^{[1
,3
,4
]}

机构：

[1] Univ Nacl Rio Cuarto, RA-5800 Cordoba, Argentina

[2] Consejo Nacl Invest Cient & Tecn, Inst Biotecnol Ambiental & Salud INBIAS, Dept Biol Mol, Fac Ciencias Exactas Fisicoquim & Nat, RA-5800 Cordoba, Argentina

[3] Consejo Nacl Invest Cient & Tecn, Fac Ciencias Exactas Fisicoquim & Nat, Dept Quim, RA-5800 Cordoba, Argentina

[4] UNRC, CONICET, IITEMA, Rio Cuarto, Argentina

[5] Consejo Nacl Invest Cient & Tecn, Dept Fis, Fac Ciencias Exactas Fisicoquim & Nat, RA-5800 Cordoba, Argentina

[6] Univ Nacl La Plata, RA-1900 Buenos Aires, DF, Argentina

[7] Consejo Nacl Invest Cient & Tecn, Dept Quim, Inst Invest Fisicoquim Teor & Aplicadas INIFTA, Fac Ciencias Exactas,CCT Plata CONICET, RA-1900 Buenos Aires, DF, Argentina

来源：

NANOMEDICINE | 2018年 / 13卷 / 06期

关键词：

apoptosis; brain tumor; colorectal tumor; conjugated polymer nanoparticles; metallated porphyrin; photodynamic therapy; ROS; SINGLET OXYGEN GENERATION; OF-THE-ART; CANCER TREATMENT; DOTS; PHOTOSENSITIZERS; COLOCALIZATION; SENSITIZERS; MECHANISMS; MULTICOLOR; ACID;

D O I：

10.2217/nnm-2017-0292

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: Assess biocompatibility, uptake and photodynamic therapy (PDT) mechanism of metallated porphyrin doped conjugated polymer nanoparticles (CPNs) in human brain and colorectal tumor cells and macrophages. Materials & methods: CPNs were developed employing 9,9-dioctylfluorene-alt-benzothiadiazole, an amphiphilic polymer (PS-PEG-COOH), and platinum octaethylporphyrin. T98G, SW480 and RAW 264.7 cell lines were exposed to CPNs to assess uptake and intracellular localization. Additionally, a PDT protocol using CPNs was employed for the in vitro killing of cancer and macrophage cell lines. Results & conclusion: CPNs were well incorporated into glioblastoma and macrophage cells with localization in lysosomes. SW480 cells were less efficient incorporating CPNs with localization in the plasma membrane. In all cell lines PDT treatment was efficient inducing oxidative stress that triggered apoptosis.

引用

页码：605 / 624

页数：20

共 68 条

[1] Fluorescence superquenching of conjugated polyelectrolytes: applications for biosensing and drug discovery [J].