The present paper investigates bacterial cellulose (BC) biosynthesis by Acetobacter xylinum DSMZ-2004, a source of microbial exploitation for cellulose obtainment, using poor quality apple extract and glycerol as carbon source. The objective of this research was to determine the influence of some significant biosynthesis medium parameters on bacterial cellulose production by enhancing the value of inadequate quality apples in a substrate combined with glycerol, leading to their superior conversion. Significant cost reduction is possible, along with an improvement in production efficiency and profitability, the lower limit of microbial cellulose cost being determined by the price of the raw matter used as a substrate. Inadequate quality apples and glycerol as a by-product of biodiesel production are not only cheap, but also abundant. It has been proven that bacterial cellulose production has been improved by applying the Taguchi experimental model of culture medium optimization, the maximum BC yield being of 8.6 g/L.