Absolute and Relative Quantification of Multiplex DNA Assays Based on an Elemental Labeling Strategy

被引：120
作者：
Han, Guojun [1 ]
Zhang, Sichun [1 ]
Xing, Zhi [1 ]
Zhang, Xinrong [1 ]
机构：
[1] Tsinghua Univ, Dept Chem, Beijing Key Lab Microanalyt Methods & Instrumenta, Beijing 100084, Peoples R China
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 05期
基金：
中国国家自然科学基金;
关键词：
absolute quantification; DNA assay; elemental labeling; mass spectrometry; rare earth elements; PLASMA-MASS SPECTROMETRY; ICP-MS; QUANTITATIVE PROTEOMICS; NANOPARTICLE PROBES; TAGGED IMMUNOASSAY; NUCLEIC-ACIDS; BIO-MOLECULES; PROTEINS; TAGS; DIAGNOSTICS;
D O I：
10.1002/anie.201206903
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Elements and quantification: A nucleic acid assay has been developed, based on an elemental labeling strategy using magnetic microparticles (MMPs), which provides quantification of multiple DNA targets. Rare-earth elements, indium, and stable isotopes could be labeled with oligonucleotides serving as DNA probes. Quantitative analysis was then carried out using the designed systems (see picture) and elemental mass spectrometry. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：1466 / 1471
页数：6
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