Detection of quorum sensing systems of bacteria isolated from fouled marine organisms

被引:19
作者
Cuadrado-Silva, Carmen T. [1 ]
Castellanos, Leonardo [1 ]
Arevalo-Ferro, Catalina [2 ]
Osorno, Oscar E. [1 ]
机构
[1] Univ Nacl Colombia, Fac Ciencias, Dept Quim, Bogota 14490, DC, Colombia
[2] Univ Nacl Colombia, Fac Ciencias, Dept Biol, Bogota 14490, DC, Colombia
关键词
Marine bacteria; Quorum sensing; Fouling; N-acyl homoserine lactones; ACYL-HOMOSERINE LACTONE; SIGNAL PRODUCTION; COMMUNICATION; IDENTIFICATION; BIOSENSORS; MOLECULES; SPONGES; SEA;
D O I
10.1016/j.bse.2012.09.010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
N-Acyl homoserine lactones (AHLs or N-AHLs) are a class of signaling molecules involved in bacterial quorum sensing (qs) that have recently been proposed as mediators of the fouling process. In this study, we determined the presence of AHLs in the following marine bacteria strains, which were collected in Santa Marta Bay (Colombia) from heavily fouled surfaces: Ochrobactrum sp., Vibrio sp. (23-6PIN), Vibrio campbellii, Vibrio sp. (11-6DEP), Ochrobactrum pseudogringnonense, Shewanella sp., Vibrio harveyi and Alteromonas sp. The detection and identification of AHLs was conducted using the microbial biosensor Escherichia coli (pSB401) and GC-MS and HPLC-MS analyses. We found that all isolated marine strains had quorum sensing systems mediated by either N-butanoyl homoserine lactone or N-hexanoyl homoserine lactone and in some cases by both. These results are in agreement with the theory that qs is involved in the fouling process. It is noteworthy to mention that we identified qs systems for the first time in bacteria of the genera Ochrobactrum and Alteromonas. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:101 / 107
页数:7
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