Purification of High-quality Plastid DNA from the Brown Alga Laminaria japonica Sporophytes

被引：1
|
作者：
Fu, Wandong [1 ,2 ]
Yao, Jianting [1 ,2 ]
Wang, Xiuliang [1 ]
Liu, Fuli [1 ,2 ]
Chong, Zhuo [1 ,2 ]
Duan, Delin [1 ]
机构：
[1] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
来源：
PLANT MOLECULAR BIOLOGY REPORTER | 2008年 / 26卷 / 02期
关键词：
Brown alga; Laminaria japonica; Plastid DNA; Ultracentrifugation;
D O I：
10.1007/s11105-008-0028-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Extracting DNA from a variety of algae is rather difficult because of high levels of polysaccharides, tannins, and phenolics as these interfere with DNA isolation and downstream applications. High-quality plastid DNA (ptDNA) purification is particularly difficult because of its small proportion in total genomic DNA. This report describes an improved protocol for ptDNA purification that efficiently produces high-quality ptDNA from sporophytes of Laminaria japonica and several other algae. This improved protocol simplifies procedures for ptDNA purification and improves yield to 150-200 mu g of ptDNA per 100 g of frozen algal tissue. Polymerase chain reaction (PCR) amplification of conserved sequences has been used to verify purity of the ptDNA product.
引用
收藏
页码：114 / 120
页数：7
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