A highly specific ELISA for diagnosis of 2009 influenza A (H1N1) virus infections

被引：14

作者：

Lu, Chun-Yi ^{[1
]}

Chang, Luan-Yin ^{[1
]}

Chen, Pei-Jer ^{[2
]}

Xia, Ning-Shao ^{[3
]}

Shao, Pei-Lan ^{[1
]}

Huang, Li-Min ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Pediat, Coll Med, Natl Taiwan Univ Hosp, Taipei 100, Taiwan

[2] Natl Taiwan Univ, Dept Internal Med, Natl Taiwan Univ Hosp, Coll Med, Taipei 100, Taiwan

[3] Xiamen Univ, Natl Inst Diagnost & Vaccine Dev Infect Dis, Minist Educ Cell Biol & Tumour Cell Engn, Key Lab, Xiamen, Peoples R China

来源：

JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION | 2012年 / 111卷 / 12期

关键词：

diagnosis; ELISA; pandemic; 2009 Influenza A (H1N1); TESTS; IMMUNOASSAY; SENSITIVITY; PCR;

D O I：

10.1016/j.jfma.2011.11.029

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background/Purpose: Accurate and timely diagnosis is vital for the clinical management of influenza. A 2009 pandemic influenza A (H1N1)-specific enzyme-linked immunosorbent assay (ELISA) was tested with selected clinical samples. Methods: A selection of 90 throat swab samples with various viral loads of 2009 pandemic influenza A (H1N1) were tested. Results: Using the reverse transcriptase polymerase chain reaction as a gold standard, the overall sensitivity (0.57) was higher than that of the QuickVue Influenza A+B Test (0.43). The specificity of the ELISA was 1.0 using the selected sample set. The positive and negative predictive values were 1 and 0.4, respectively. Conclusion: The ELISA is an easy to perform, highly specific, and fairly sensitive diagnostic tool for the 2009 pandemic influenza A (H1N1) virus infections. A strong correlation was found between viral load and specificity. Copyright (C) 2012, Elsevier Taiwan LLC & Formosan Medical Association. All rights reserved.

引用

页码：693 / 697

页数：5

共 12 条

[1]

[Anonymous], 2011, WKLY EPIDEMIOL REC, V86, P222

[2] Rapid clinical diagnosis in fatal swine influenza (H1N1) pneumonia in an adult with negative rapid influenza diagnostic tests (RIDTs): Diagnostic swine influenza triad [J].

Cunha, Burke A. ;

Syed, Uzma ;

Mickail, Nardeen ;

Strollo, Stephanie .

HEART & LUNG, 2010, 39 (01) :78-86

[3] Rapid-Test Sensitivity for Novel Swine-Origin Influenza A (H1N1) Virus in Humans [J].

Faix, Dennis J. ;

Sherman, Sterling S. ;

Waterman, Steven H. .

NEW ENGLAND JOURNAL OF MEDICINE, 2009, 361 (07) :728-729

[4] Performance of six influenza rapid tests in detecting human influenza in clinical specimens [J].

Hurt, Aeron C. ;

Alexander, Robert ;

Hibbert, Jan ;

Deed, Nicola ;

Barr, Ian G. .

JOURNAL OF CLINICAL VIROLOGY, 2007, 39 (02) :132-135

[5]

Panning M, 2009, EUROSURVEILLANCE, V14, P7

[6] Comparison of the directigen Flu A+B test, the QuickVue influenza test, and clinical case definition to viral culture and reverse transcription-PCR for rapid diagnosis of influenza virus infection [J].

Ruest, A ;

Michaud, S ;

Deslandes, S ;

Frost, EH .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (08) :3487-3493

[7] Viral Load in Patients Infected With Pandemic H1N1 2009 Influenza A Virus [J].

To, Kelvin K. W. ;

Chan, Kwok-Hung ;

Li, Iris W. S. ;

Tsang, Tak-Yin ;

Tse, Herman ;

Chan, Jasper F. W. ;

Hung, Ivan F. N. ;

Lai, Sik-To ;

Leung, Chi-Wai ;

Kwan, Yat-Wah ;

Lau, Yu-Lung ;

Ng, Tak-Keung ;

Cheng, Vincent C. C. ;

Peiris, Joseph S. M. ;

Yuen, Kwok-Yung .

JOURNAL OF MEDICAL VIROLOGY, 2010, 82 (01) :1-7

[8] Rapid Antigen Tests for Diagnosis of Pandemic (Swine) Influenza A/H1N1 [J].

Vasoo, Shawn ;

Stevens, Jane ;

Singh, Kamaljit .

CLINICAL INFECTIOUS DISEASES, 2009, 49 (07) :1090-1093

[9] Evaluation of QuickVue influenza A plus B rapid test for detection of pandemic influenza A/H1N1 2009 [J].

Velasco, John Mark S. ;

Montesa-Develos, Maribel L. ;

Jarman, Richard G. ;

Lopez, Ma. Nila B. ;

Gibbons, Robert V. ;

Valderama, Maria Theresa G. ;

Yoon, In-Kyu .

JOURNAL OF CLINICAL VIROLOGY, 2010, 48 (02) :120-122

[10] Sensitivity of rapid immunoassay for influenza A and B in the early phase of the disease [J].

Watanabe, Masahiro ;

Nakagawa, Naoko ;

Ito, Masahiro ;

Ihara, Toshiaki .

PEDIATRICS INTERNATIONAL, 2009, 51 (02) :211-215

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