Stratification of Archaea in the Deep Sediments of a Freshwater Meromictic Lake: Vertical Shift from Methanogenic to Uncultured Archaeal Lineages

被引：61

作者：

Borrel, Guillaume ^{[1
,2
]}

Lehours, Anne-Catherine ^{[1
,2
]}

Crouzet, Olivier ^{[1
,2
]}

Jezequel, Didier ^{[3
]}

Rockne, Karl ^{[4
]}

Kulczak, Amelie ^{[1
,2
]}

Duffaud, Emilie ^{[1
,2
]}

Joblin, Keith ^{[1
,2
]}

Fonty, Gerard ^{[1
,2
]}

机构：

[1] Univ Blaise Pascal, Clermont Univ, UMR CNRS 6023, Lab Microorganismes Genome & Environm, Aubiere, France

[2] Univ Auvergne, Aubiere, France

[3] Univ Paris 07, UMR CNRS 7154, Inst Phys Globe Paris, Lab Geochim Eaux, Paris, France

[4] Univ Illinois, Dept Civil & Mat Engn, Chicago, IL USA

来源：

PLOS ONE | 2012年 / 7卷 / 08期

关键词：

GRADIENT GEL-ELECTROPHORESIS; POLYMERASE-CHAIN-REACTION; MARINE BENTHIC ARCHAEA; RIBOSOMAL-RNA GENES; COMMUNITY STRUCTURE; MICROBIAL COMMUNITIES; ANAEROBIC OXIDATION; CRATER LAKE; HYPERSALINE LAKE; LAC-PAVIN;

D O I：

10.1371/journal.pone.0043346

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

As for lineages of known methanogens, several lineages of uncultured archaea were recurrently retrieved in freshwater sediments. However, knowledge is missing about how these lineages might be affected and structured according to depth. In the present study, the vertical changes of archaeal communities were characterized in the deep sediment of the freshwater meromictic Lake Pavin. For that purpose, an integrated molecular approach was performed to gain information on the structure, composition, abundance and vertical stratification of archaeal communities thriving in anoxic freshwater sediments along a gradient of sediments encompassing 130 years of sedimentation. Huge changes occurred in the structure and composition of archaeal assemblages along the sediment core. Methanogenic taxa (i.e. Methanosaeta and Methanomicrobiales) were progressively replaced by uncultured archaeal lineages (i.e. Marine Benthic Group-D (MBG-D) and Miscellaneous Crenarchaeal Group (MCG)) which are suspected to be involved in the methane cycle.

引用

页数：14

共 84 条

[11] Production and consumption of methane in freshwater lake ecosystems [J].