Capillary Electrophoresis with Laser-induced Fluorescence Detection for Application in Intracellular Investigation of Anthracyclines and Multidrug Resistance Proteins

被引：9

作者：

Mbuna, Julius ^{[1
]}

Kaneta, Takashi ^{[2
]}

机构：

[1] Univ Dar Es Salaam, Coll Educ, Dept Chem, Dar Es Salaam, Tanzania

[2] Okayama Univ, Grad Sch Nat Sci & Technol, Dept Chem, Okayama 7008530, Japan

来源：

ANALYTICAL SCIENCES | 2015年 / 31卷 / 11期

关键词：

Capillary electrophoresis; micellar electrokinetic chromatography; capillary electrophoresis immunoassay; laser-induced fluorescence; anthracycline; multidrug resistance associated protein; cancer cell; P-GLYCOPROTEIN; SUBCELLULAR-LOCALIZATION; ABSOLUTE QUANTIFICATION; LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; HUMAN URINE; CELL-LINES; DOXORUBICIN; TRANSPORTER; ACCUMULATION;

D O I：

10.2116/analsci.31.1121

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary electrophoresis (CE) coupled with laser-induced fluorescence (LIF) is a powerful method for the trace analysis of cellular components. This review presents a summary of topics for the direct analysis of anthracyclines and multidrug resistance proteins in cancerous cells. A micellar electrokinetic chromatography (MEKC) method that does not use organic solvents, and hence prevents the precipitation of proteins in cellular samples, was shown to be a reliable method for the determination of several anthracyclines including the epimeric doxorubicin and epirubicin. A fast CE-based immunoassay for investigating transporter multidrug resistance associated protein (MRP1) was also developed for routine or explorative analysis of the levels of transporter proteins in cancerous cells. A combination of the developed MEKC-LIF method and the CE immunoassay (CEIA) method has permitted the analysis of anthracyclines and MRP1 in a cell line to show the relationship between the levels of MRP1 and amount of anthracyclines in cancerous cells.

引用

页码：1121 / 1128

页数：8

共 78 条

[1] Simultaneous determination of anthracyclines and taxanes in human serum using online sample extraction coupled to high performance liquid chromatography with UV detection [J].

Bermingham, Shane ;

O'Connor, Robert ;

Regan, Fiona ;

McMahon, Gillian P. .

JOURNAL OF SEPARATION SCIENCE, 2010, 33 (11) :1571-1579

[2] Mammalian ABC transporters in health and disease [J].

Borst, P ;

Elferink, RO .

ANNUAL REVIEW OF BIOCHEMISTRY, 2002, 71 :537-592

[3] Selective determination of the doxorubicin content of individual acidic organelles in impure subcellular fractions [J].

Chen, Y ;

Walsh, RJ ;

Arriaga, EA .

ANALYTICAL CHEMISTRY, 2005, 77 (08) :2281-2287

[4] Micellar electrokinetic chromatography profiles of human high-density lipoprotein phospholipids [J].

Chong, Chin-Pong ;

Lin, Ting-Yu ;

Chang, Chia-Liang ;

Yang, Ying-Ling ;

Tsai, Ming-Hua ;

Yu, Yu-Shan ;

Liu, Mine-Yine .

ELECTROPHORESIS, 2011, 32 (10) :1241-1251

[5] Mutations in the MDR3 gene cause progressive familial intrahepatic cholestasis [J].

De Vree, JML ;

Jacquemin, E ;

Sturm, E ;

Cresteil, D ;

Bosma, PJ ;

Aten, J ;

Deleuze, JF ;

Desrochers, M ;

Burdelski, M ;

Bernard, O ;

Elferink, RPJO ;

Hadchouel, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (01) :282-287

[6] The human ATP-binding cassette (ABC) transporter superfamily [J].

Dean, M ;

Rzhetsky, A ;

Allikmets, R .

GENOME RESEARCH, 2001, 11 (07) :1156-1166

[7] Evaluation and comparison of MRP1 activity with three fluorescent dyes and three modulators in leukemic cell lines [J].

Dogan, AL ;

Legrand, O ;

Faussat, AM ;

Perrot, JY ;

Marie, JP .

LEUKEMIA RESEARCH, 2004, 28 (06) :619-622

[8] A multidrug resistance transporter from human MCF-7 breast cancer cells [J].

Doyle, LA ;

Yang, WD ;

Abruzzo, LV ;

Krogmann, T ;

Gao, YM ;

Rishi, AK ;

Ross, DD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (26) :15665-15670

[9] A capillary electrophoresis laser-induced fluorescence method for analysis of potato glycoalkaloids based on a solution-phase immunoassay. 1. Separation and quantification of immunoassay products [J].

Driedger, DR ;

LeBlanc, RJ ;

LeBlanc, EL ;

Sporns, P .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (04) :1135-1139

[10] Electrochemical immunoassay of membrane P-glycoprotein by immobilization of cells on gold nanoparticles modified on a methoxysilyl-terminated butyrylchitosan matrix [J].

Du, D ;

Ju, HX ;

Zhang, XJ ;

Chen, J ;

Cai, J ;

Chen, HY .

BIOCHEMISTRY, 2005, 44 (34) :11539-11545

← 1 2 3 4 5 6 7 8 →