NUCLEOTIDE-SEQUENCE;
CHINESE ISOLATE;
CDNA;
AGROINFECTION;
VERSATILE;
PLANTS;
RNA;
D O I:
10.1007/s00705-013-1625-7
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5' end of both RNA1 and RNA2 starts with a GUU sequence. We successfully inoculated Nicotiana benthamiana with BBWV-2 by infiltrating a mixed suspension of two Agrobacterium tumefaciens clones carrying binary vectors with the full-length RNA1 and RNA2 sequences. This is the first report on the efficient, easy and high-throughput use of agroinoculation for generating BBWV-2 infections.