Two molluscan BCL-2 family members from Manila clam, Ruditapes philippinarum: Molecular characterization and immune responses

被引：11
|
作者：
Lee, Youngdeuk [1 ]
Whang, Ilson [2 ]
Lee, Sukkyoung [2 ]
Menike, Udeni [3 ]
Oh, Chulhong [1 ]
Kang, Do-Hyung [1 ]
Heo, Gang-Joon [4 ]
Lee, Jehee [2 ]
De Zoysa, Mahanama [3 ]
机构：
[1] Korea Inst Ocean Sci Technol, Ansan 426744, South Korea
[2] Jeju Natl Univ, Dept Marine Life Sci, Cheju 690756, South Korea
[3] Chungnam Natl Univ, Inst Vet Med, Coll Vet Med & Res, Taejon 305764, South Korea
[4] Chungbuk Natl Univ, Coll Vet Med, Cheongju 361763, South Korea
来源：
FISH & SHELLFISH IMMUNOLOGY | 2013年 / 34卷 / 06期
基金：
新加坡国家研究基金会;
关键词：
Apoptosis; B cell lymphoma 2 (BCL-2); Manila clam; Ruditapes philippinarum; Vibrio tapetis; MITOCHONDRIAL PATHWAY; APOPTOSIS; DEATH; EXPRESSION; STRESS; COMPLEXITY; EVOLUTION; ALIGNMENT; LIFE;
D O I：
10.1016/j.fsi.2013.03.366
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Apoptosis based immune responses are important component of host defense in mollusks. In this study, we have identified two novel molluscan BCL-2 cDNAs from Manila clam, Ruditapes philippinarum and named as RpBCL-2A and RpBCL-2B. There were four and three highly conserved BCL-2 homology (BH) regions in RpBCL-2A and RpBCL-2B, respectively suggesting these two genes could be different isoforms of anti-apoptotic BCL-2 family. Phylogenetic results revealed that Manila clam BCL-2 genes were clustered closely with invertebrate BCL-2 members. It gives evidence of their common origin and conserved features of invertebrate BCL-2 family. RpBCL-2A and 2B were expressed in tissue-specific manner showing the highest and lowest level of expression in gills and hemocytes, respectively. However there was no clear expression profile difference between two genes. After Vibrio tapetis challenge, transcriptional responses of RpBCL-2A and RpBCL-2B were induced in gills and hemocytes with high variation that could be due to effects of immune reactions of other host defense molecules. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1628 / 1634
页数：7
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