Microheterogeneity and microrheology of wheat gliadin suspensions studied by multiple-particle tracking

被引:43
作者
Xu, JY
Tseng, Y
Carriere, CJ
Wirtz, D
机构
[1] Johns Hopkins Univ, Dept Chem Engn, Grad Program Mol Biophys, Baltimore, MD 21218 USA
[2] Johns Hopkins Univ, Dept Mat Sci & Engn, Baltimore, MD 21218 USA
[3] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA
关键词
D O I
10.1021/bm015586b
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
By monitoring the thermally driven displacements of imbedded polystyrene microspheres via video fluorescence microscopy, we quantified the microstructural and micromechanical heterogeneities of wheat gliadin suspensions. We found that the degree of heterogeneity of the suspensions, as measured by the width and skewness of the microspheres' mean squared displacement (MSD) distribution, increased dramatically over a narrow range of gliadin concentrations. The ensemble-averaged MSD of a 250 mg/mL gliadin suspension exhibited a power-law behavior scaling linearly with time, a behavior similar to that observed for a homogeneous aqueous glycerol solution. However, the MSD distribution was wider and more asymmetric than for glycerol. With increasing concentration of gliadin, the ensemble-averaged MSD rapidly displayed a plateau at small time scales, the MSD distribution became wider and more asymmetric, and the local viscoelastic moduli extracted from multiple-particle-tracking measurements showed an increasingly wide range.
引用
收藏
页码:92 / 99
页数:8
相关论文
共 44 条
[21]   A 'hot-spot' mutation alters the mechanical properties of keratin filament networks [J].
Ma, LL ;
Yamada, S ;
Wirtz, D ;
Coulombe, PA .
NATURE CELL BIOLOGY, 2001, 3 (05) :503-506
[22]  
MacRitchie F., 1992, Advances in Food and Nutrition Research, V36, P1, DOI 10.1016/S1043-4526(08)60104-7
[23]  
Mason TG, 1997, MAT RES S C, V463, P153
[24]   Particle tracking microrheology of complex fluids [J].
Mason, TG ;
Ganesan, K ;
vanZanten, JH ;
Wirtz, D ;
Kuo, SC .
PHYSICAL REVIEW LETTERS, 1997, 79 (17) :3282-3285
[25]   Diffusing wave spectroscopy microrheology of actin filament networks [J].
Palmer, A ;
Mason, TG ;
Xu, JY ;
Kuo, SC ;
Wirtz, D .
BIOPHYSICAL JOURNAL, 1999, 76 (02) :1063-1071
[26]  
Palmer A, 1998, J POLYM SCI POL PHYS, V36, P3007, DOI 10.1002/(SICI)1099-0488(199812)36:17<3007::AID-POLB1>3.0.CO
[27]  
2-D
[28]   IDENTIFICATION OF A HIGH-MOLECULAR-WEIGHT SUBUNIT OF GLUTENIN WHOSE PRESENCE CORRELATES WITH BREAD-MAKING QUALITY IN WHEATS OF RELATED PEDIGREE [J].
PAYNE, PI ;
CORFIELD, KG ;
BLACKMAN, JA .
THEORETICAL AND APPLIED GENETICS, 1979, 55 (3-4) :153-159
[29]   Reversible hydrogels from self-assembling artificial proteins [J].
Petka, WA ;
Harden, JL ;
McGrath, KP ;
Wirtz, D ;
Tirrell, DA .
SCIENCE, 1998, 281 (5375) :389-392
[30]  
PRESTON K, 1975, CEREAL CHEM, V52, P427