Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams

被引：3
|
作者：
Costa, L. F. [1 ]
Nozaki, C. N. [1 ]
Lira, N. S. C. [1 ]
Antunes, J. M. A. P. [1 ]
Xavier, M. N. [2 ]
Costa, E. A. [2 ]
Paixao, T. A. [2 ]
Santos, R. L. [2 ]
Megid, J. [1 ]
机构：
[1] Fac Med Vet & Zootecnia, Botucatu, SP, Brazil
[2] Univ Fed Minas Gerais, Escola Vet, Belo Horizonte, MG, Brazil
来源：
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA | 2013年 / 65卷 / 01期
基金：
巴西圣保罗研究基金会;
关键词：
Brucella ovis; species-specific; nested PCR; semen; urine; GENUS BRUCELLA; DNA; ABORTUS; FETUSES;
D O I：
10.1590/S0102-09352013000100009
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams. The performance of the species-specific nested PCR was compared with the results of a genus-specific PCR. Fourteen rams were experimentally infected with the Brucella ovis REO 198 strain and samples of semen and urine were collected every week up to 180 days post infection. Out of 83 semen samples collected, 42 (50.6%) were positive for the species-specific nested PCR, and 23 (27.7%) were positive for the genus-specific PCR. Out of 75 urine samples, 49 (65.3%) were positive for the species-specific nested PCR, whereas 11 (14.6%) were genus-specific PCR positive. Species-specific nested PCR was significantly more sensitive (P<0.001) than the genus-specific PCR in semen and urine from experimentally infected rams. In conclusion, the species-specific nested PCR developed in this study may be used as a diagnostic tool for the detection of B. ovis in semen and urine samples from suspected rams.
引用
收藏
页码：55 / 60
页数：6
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