Enhanced production of human epidermal growth factor by a recombinant Escherichia coli integrated with in situ exchange of acetic acid by macroporous ion-exchange resin

被引：13
作者：
Chen, XA
Cen, PL
Chen, JS
机构：
[1] Zhejiang Univ, Dept Chem Engn & Bioengn, Inst Bioengn, Hangzhou 310027, Peoples R China
[2] Zhejiang Univ, Coll Life Sci, Zhejiang 310029, Peoples R China
来源：
JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2005年 / 100卷 / 05期
关键词：
in situ exchange; recombinant Escherichia coli; human epidermal growth factor (hEGF) production; ion-exchange resin;
D O I：
10.1263/jbb.100.579
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A macroporous ion-exchange resin was adopted for the in situ exchange of acetic acid during human epidermal growth factor (hEGF) production by recombinant Escherichia coli JM101. The results of this study suggest that in situ exchange by macroporous resin can improve E. coli growth, decrease acetate accumulation and enhance hEGF production.
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收藏
页码：579 / 581
页数：3
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